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Detection of circulating tumour cells in the breast cancer using CytoTrack system
- Source :
- Herba Polonica, Vol 65, Iss 4, Pp 31-36 (2019)
- Publication Year :
- 2019
- Publisher :
- Walter de Gruyter GmbH, 2019.
-
Abstract
- Summary Introduction: Plants are a rich source of healing substances. Cancer is a leading cause of death worldwide while breast cancer is the most common cancer among women. Circulating tumour cells (CTCs) are potential founder cells for metastasis. Therefore, their assessment may be used for monitoring of treatment as well as detecting cancer metastatis. Hence, it is suggested that the number of CTCs may be a valuable tumour biomarker during therapy. Objective: The purpose of this study was to detect CTCs in breast cancer and to validate the method of assessment of CTC count using CytoTrack CT11 technology. Methods: MCF-7 cells were sorted by a FACSARIA flow cytometer from blood samples derived from patients who have not been diagnosed with cancer. Identification and quantitative assessment of MCF-7 cells in blood samples were determined by flow sorting. Then, blood samples containing MCF-7 cells or without MCF-7 were scanned with the use of an automated fluorescence scanning microscope. Results: In in vitro model analysing the glass CytoDisc™ with stained MCF-7 cells, we noted the correlation between the amount of observed tumour cells and expected number of tumour cells. Moreover, coefficient of variation in case of the recovery rate of the assumed number of MCF-7 cells was 30%, 17%, 18% and 15%, respectively. Conclusion: Our study suggest that CTCs could be predictive factor in patients with metastatic cancer especially in breast cancer.
- Subjects :
- 0303 health sciences
liquid biopsy
circulating tumour cells
business.industry
Plant culture
Cancer
medicine.disease
SB1-1110
Predictive factor
Metastasis
cytotrack ct11
03 medical and health sciences
breast cancer
0302 clinical medicine
Breast cancer
030220 oncology & carcinogenesis
Cancer research
Quantitative assessment
medicine
Biomarker (medicine)
In patient
business
030304 developmental biology
Cause of death
Subjects
Details
- ISSN :
- 24498343
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Herba Polonica
- Accession number :
- edsair.doi.dedup.....60ac2af4f57b5ce519a627856716f703