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LC-MS/MS profiling-based secondary metabolite screening of Myxococcus xanthus
- Source :
- Journal of microbiology and biotechnology. 19(1)
- Publication Year :
- 2009
-
Abstract
- Myxobacteria, Gram-negative soil bacteria, are a well-known producer of bioactive secondary metabolites. Therefore, this study presents a methodological approach for the high-throughput screening of secondary metabolites from 4 wild-type Myxococcus xanthus strains. First, electrospray ionization mass spectrometry (ESI-MS) was performed using extracellular crude extracts. As a result, 22 metabolite peaks were detected, and the metabolite profiling was then conducted using the m/z value, retention time, and MS/MS fragmentation pattern analyses. Among the peaks, one unknown compound peak was identified as analogous to the myxalamid A, B, and C series. An analysis of the tandem mass spectrometric fragmentation patterns and HR-MS identified myxalamid K as a new compound derived from M. xanthus. In conclusion, LC-MS/MS-based chemical screening of diverse secondary metabolites would appear to be an effective approach for discovering unknown microbial secondary metabolites.
- Subjects :
- Myxococcus xanthus
Spectrometry, Mass, Electrospray Ionization
Chromatography
biology
Metabolite
Electrospray ionization
General Medicine
Polyenes
Secondary metabolite
biology.organism_classification
Tandem mass spectrometry
Mass spectrometry
Applied Microbiology and Biotechnology
Anti-Bacterial Agents
chemistry.chemical_compound
chemistry
Myxobacteria
Tandem Mass Spectrometry
Extracellular
medicine
Biotechnology
medicine.drug
Chromatography, Liquid
Subjects
Details
- ISSN :
- 10177825
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of microbiology and biotechnology
- Accession number :
- edsair.doi.dedup.....6094062c302c07d7335654bd63caf5bc