Back to Search
Start Over
Curcumin Regulates the Progression of Colorectal Cancer via LncRNA NBR2/AMPK Pathway
- Source :
- Technology in Cancer Research & Treatment
- Publication Year :
- 2019
- Publisher :
- SAGE Publications, 2019.
-
Abstract
- Objective: To identify the effect of curcumin on tumor suppression and the possible molecular pathways involved. Methods: The expression of long noncoding RNA neighbor of BRCA1 lncRNA 2 (NBR2) was quantified using reverse transcription-polymerase chain reaction on cultured colorectal cancer cells. Next, we used Western blot to measure the activation of adenosine monophosphate-activated protein kinase and mechanistic target of rapamycin kinase (mTOR) signaling molecules. Both cell proliferation and viability were measured via MTT assay, and the cell ratio and S phase were detected by BrdU assay. Colorectal cancer cells were pretreated with curcumin or transfected with shNBR2 or adenosine monophosphate-activated protein kinase inhibitor Compound C to examine the molecular pathway involved. Results: Current data showed that glucose deficiency increased the expression of NBR2 in colorectal cancer cells, and NBR2 knockdown affected the progression of colorectal cancer cells under glucose starvation conditions. When NBR2 was silenced in the treated colorectal cancer cells, the proliferation, the clone formation, and the percentage of S-phase cells suppressed by glucose deprivation were compromised. Furthermore, NBR2 knockdown could suppress glucose deprivation-induced adenosine monophosphate-activated protein kinase activation plus mTOR inactivation. Similarly, when colorectal cancer cells were treated with curcumin, the expression of NBR2 was significantly increased. NBR2 knockdown reversed curcumin-suppressed proliferation, clone formation, and the percentage of S-phase colorectal cancer cells. Furthermore, NBR2 knockdown abolished curcumin-induced activation of adenosine monophosphate-activated protein kinase and inactivation of the mTOR signaling pathway. Conclusion: This study revealed a novel mechanism by which long noncoding RNA NBR2 mediates curcumin suppression of colorectal cancer proliferation by activating adenosine monophosphate-activated protein kinase and inactivating the mTOR signaling pathway.
- Subjects :
- AMPK
Cancer Research
Colorectal cancer
colorectal cancer
AMP-Activated Protein Kinases
Biology
Gene Knockout Techniques
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line, Tumor
medicine
Humans
curcumin
Cell Proliferation
030304 developmental biology
0303 health sciences
TOR Serine-Threonine Kinases
NBR2
medicine.disease
Antineoplastic Agents, Phytogenic
Long non-coding RNA
Gene Expression Regulation, Neoplastic
Glucose
Oncology
chemistry
030220 oncology & carcinogenesis
Disease Progression
Curcumin
Cancer research
RNA, Long Noncoding
Original Article
Colorectal Neoplasms
Signal Transduction
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 15330338 and 15330346
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Technology in Cancer Research & Treatment
- Accession number :
- edsair.doi.dedup.....608e3f0d16bdf04babac2552c606cc5c