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Morphine modulation of plasmodial-antigens-induced colony-stimulating factors production by macrophages
- Source :
- Life sciences. 67(9)
- Publication Year :
- 2000
-
Abstract
- Morphine abuse is known to cause immunosuppression and enhanced host susceptibility to malaria. We studied the effect of morphine on the Plasmodium berghei total-parasite-antigens soluble in culture medium (P.b.SA)-induced production of colony-stimulating factors (CSFs) by mouse peritoneal macrophages, in vitro. Morphine exerted a concentration-dependent biphasic modulatory effect; at 1 x 10(-4)-1 x 10 x 10(-6) M it slightly inhibited, whereas at 1 X 10(-8)-1 x 10(-10) M it augmented the production of CSFs. However, at 1 x 10(-12) M concentration the augmenting effect of morphine was significantly (p0.05) diminished. Selective agonists of delta- (DPDPE) and mu- (DAGO) opioid receptors also respectively, inhibited and augmented the production of CSFs. The CSFs appear to be synthesized de novo as cycloheximide (50.0 microg/ml) completely inhibited their production. Naloxone ( 1 x 10(-5) M) lacked any effect on the inhibitory effect of morphine; however, at 1 x 10(-3) M it exerted partial blocking effect. Conversely, at 1 x 10(-5) M naloxone significantly (p0.05) blocked the augmenting effect of morphine. These results suggest that morphine via opioid receptors, in a concentration-dependent biphasic manner, modulated the P.b.SA-induced de novo production of CSFs by macrophages, in vitro.
- Subjects :
- Male
Plasmodium berghei
Narcotic Antagonists
Dose-Response Relationship, Immunologic
Receptors, Opioid, mu
Antigens, Protozoan
(+)-Naloxone
Cycloheximide
Pharmacology
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Mice
Adjuvants, Immunologic
Colony-Stimulating Factors
Receptors, Opioid, delta
medicine
Animals
Drug Interactions
General Pharmacology, Toxicology and Pharmaceutics
Receptor
Protein Synthesis Inhibitors
Mice, Inbred BALB C
biology
Morphine
business.industry
Naloxone
General Medicine
Enkephalin, Ala(2)-MePhe(4)-Gly(5)
biology.organism_classification
Colony-stimulating factor
In vitro
Analgesics, Opioid
Kinetics
chemistry
Opioid
Macrophages, Peritoneal
Female
business
Enkephalin, D-Penicillamine (2,5)
medicine.drug
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 67
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....6079c93b582f7ea843f8963e7eb15b2d