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Murine Bronchoalveolar Lavage
- Source :
- BIO-PROTOCOL. 7
- Publication Year :
- 2017
- Publisher :
- Bio-Protocol, LLC, 2017.
-
Abstract
- A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-linked immunosorbent assay (ELISA), luminex assays, etc., to examine the immune cells, pathogens, proteins such as cytokines/chemokines, and the expression levels of inflammation-related and other genes in the cells. This will help to understand the underlying mechanisms of these lung diseases and develop specific and effective drugs.
- Subjects :
- 0301 basic medicine
Chemokine
medicine.diagnostic_test
Strategy and Management
Mechanical Engineering
Metals and Alloys
respiratory system
Biology
Cell sorting
Molecular biology
Article
Industrial and Manufacturing Engineering
respiratory tract diseases
Flow cytometry
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Immune system
Bronchoalveolar lavage
030220 oncology & carcinogenesis
medicine
biology.protein
Bicinchoninic acid assay
Bradford protein assay
Immunostaining
Subjects
Details
- ISSN :
- 23318325
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- BIO-PROTOCOL
- Accession number :
- edsair.doi.dedup.....60765a35213a3d2228f93a66764e1d2e