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Ocotea glomerata (Nees) Mez Extract and Fractions: Chemical Characterization, Anti-Candida Activity and Related Mechanism of Action
- Source :
- Antibiotics, Volume 9, Issue 7, Antibiotics, Vol 9, Iss 394, p 394 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Background: Opportunistic fungal infections are increasingly common, with Candida albicans being the most common etiological agent<br />however, in recent years, episodes of candidiasis caused by non-albicans Candida species have emerged. Plants belonging to the Lauraceae family have shown remarkable antifungal effects. This study assessed the anti-Candida activity of Ocotea glomerata extracts and fractions, time of death and the synergistic effects with conventional antifungals. The possible mechanism of action was also addressed. Methods: Minimal inhibitory concentrations (MIC) were determined by broth microdilution technique, and the mechanism of action was assessed by ergosterol, sorbitol, cell viability, reactive oxygen species (ROS) generation and phosphatidylserine externalization tests. Results: All the tested extracts evidenced antifungal activity, but the methanol extract was revealed to be the most effective (MIC = 3.12 &mu<br />g/mL) on C. krusei. The combination of methanol extract with ketoconazole and fluconazole revealed a synergistic effect for C. krusei and C. albicans, respectively. Fractions 1 and 5 obtained from the methanol extract had fungicidal activity, mainly against C. krusei. Methanol extract did not reveal effects by ergosterol and sorbitol assays<br />however, it led to an increase in intracellular ROS levels, decreased cell viability, and consequently, cell death. Conclusion: O. glomerata methanol extract may be viewed as a rich source of biomolecules with antifungal activity against Candida spp.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Ocotea glomerata
natural products
030106 microbiology
Mechanism of action
Anti-candida activity
Biochemistry
Microbiology
anti-candida activity
Article
03 medical and health sciences
chemistry.chemical_compound
parasitic diseases
medicine
Pharmacology (medical)
Viability assay
General Pharmacology, Toxicology and Pharmaceutics
Candida albicans
Ergosterol
Natural products
biology
Traditional medicine
Broth microdilution
lcsh:RM1-950
biology.organism_classification
Corpus albicans
030104 developmental biology
Infectious Diseases
lcsh:Therapeutics. Pharmacology
chemistry
Sorbitol
Ketoconazole
Fluconazole
medicine.drug
mechanism of action
Subjects
Details
- Language :
- English
- ISSN :
- 20796382
- Database :
- OpenAIRE
- Journal :
- Antibiotics
- Accession number :
- edsair.doi.dedup.....60440291829aa1dd5eecb24baceb2e82
- Full Text :
- https://doi.org/10.3390/antibiotics9070394