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Altered Expression of Circadian Clock Genes in Human Chronic Myeloid Leukemia
- Source :
- Journal of Biological Rhythms. 26:136-148
- Publication Year :
- 2011
- Publisher :
- SAGE Publications, 2011.
-
Abstract
- Circadian clock genes use transcriptional-translational feedback loops to control circadian rhythms. Recent studies have demonstrated that expression of some circadian clock genes displays daily oscillation in peripheral tissues including peripheral blood and bone marrow. Circadian rhythms regulate various functions of human body, and the disruption of circadian rhythm has been associated with cancer development and tumor progression. However, the direct links between aberrant circadian clock gene expression and human disorders remain largely unknown. In this study, comparisons were made between the expression profiles of 9 circadian clock genes from peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells (PMNs) from 18 healthy volunteers. Peripheral blood (PB) total leukocytes from 54 healthy volunteers and 95 patients with chronic myeloid leukemia (CML) were also investigated. Similar expression profiles of all 9 circadian clock genes were observed in PBMCs and PMNs of healthy individuals. In PB total leukocytes of healthy individuals, the daily pattern of PER1, PER2, PER3, CRY1, CRY2, and CKIε expression level peaked at 0800 h, and BMAL1 peaked at 2000 h. Daily pattern expression of these 7 genes was disrupted in newly diagnosed pre—imatinib mesylate—treated and blast crisis—phase patients with CML. Partial daily pattern gene expression recoveries were observed in patients with CML with complete cytogenetic response and major molecular response. The expression of CLOCK and TIM did not show a time-dependent variation among the healthy and patients with CML. These results indicate a possible association of the disrupted daily patterns of circadian clock gene expression with the pathogenesis of CML.
- Subjects :
- Adult
Male
medicine.medical_specialty
Neutrophils
Physiology
Circadian clock
Down-Regulation
Biology
Peripheral blood mononuclear cell
RAR-related orphan receptor alpha
Circadian Clocks
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Physiology (medical)
Internal medicine
medicine
Humans
Circadian rhythm
Gene Expression Regulation, Leukemic
Gene Expression Profiling
Nuclear Proteins
Myeloid leukemia
Circadian Rhythm
CLOCK
Endocrinology
medicine.anatomical_structure
Imatinib mesylate
Immunology
Leukocytes, Mononuclear
Female
Bone marrow
Subjects
Details
- ISSN :
- 15524531 and 07487304
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Rhythms
- Accession number :
- edsair.doi.dedup.....60191b899d27903b654af8a3dce18a97