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The inhibition of enterovirus 71 induced apoptosis by Durvillaea antarctica through P53 and STAT1 signaling pathway
- Source :
- Journal of Medical Virology. 93:3532-3538
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- The infection of enterovirus 71 (EV71) resulted in hand, foot, and mouth disease and may lead to severe nervous system damage and even fatalities. There are no effective drugs to treat the EV71 virus and it is crucial to find novel drugs against it. Polysaccharide isolated from Durvillaea antarctica green algae has an antiviral effect. In this study, D. antarctica polysaccharide (DAPP) inhibited the infection of EV71 was demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), reverse transcription polymerase chain reaction, flow cytometry, and western blot. MTT assay showed that DAPP had no toxicity on Vero cells at the concentration 250 μg/ml. Furthermore, DAPP significantly reduced the RNA level of EV71 in a dose-dependent manner. Moreover, DAPP inhibited the Vero cells apoptosis induced by EV71 via the P53 signaling pathway. Meanwhile, the expression of signal transducer and activator of transcription 1 and mammalian target of rapamycin were increased and the proinflammatory cytokines were significantly inhibited by DAPP. Taken together, these results suggested that DAPP could be a potential pharmaceutical against the infection of EV71 virus.
- Subjects :
- Apoptosis
Antiviral Agents
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
Western blot
Chlorophyta
Polysaccharides
Virology
Chlorocebus aethiops
medicine
Animals
MTT assay
030212 general & internal medicine
STAT1
Vero Cells
Biological Products
medicine.diagnostic_test
biology
Chemistry
Genes, p53
Molecular biology
Enterovirus A, Human
Reverse transcription polymerase chain reaction
STAT1 Transcription Factor
Infectious Diseases
Vero cell
biology.protein
STAT protein
RNA, Viral
030211 gastroenterology & hepatology
Signal Transduction
Subjects
Details
- ISSN :
- 10969071 and 01466615
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Virology
- Accession number :
- edsair.doi.dedup.....5ffb80e1115dd646ebb8fbb8e6663415