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RUNX3 protein is overexpressed in human epithelial ovarian cancer
- Source :
- Gynecologic Oncology. 112:325-330
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- RUNX family genes, including RUNX3, are developmental regulators that are important in human cancers. The purpose of this study was to evaluate expression and oncogenic potential of RUNX3 in ovarian carcinoma.Immunohistochemical staining was performed on 60 malignant, 14 borderline, and 5 normal ovarian specimens. Correlation between RUNX3 expression with tumor histology was performed. RUNX3 expression was evaluated by quantitative real-time polymerase chain reaction (QRT-PCR) in microdissected normal and malignant epithelial ovarian tissues. Cell proliferation and viability studies were performed on cells expressing RUNX3 by lentiviral infection and cells with silenced RUNX3 expression by siRNA.RUNX3 expression by immunohistochemistry was higher in serous ovarian carcinomas versus normal ovarian epithelium (P0.001). Immunofluorescent staining confirmed upregulation of cytoplasmic RUNX3 in ovarian cancer cell lines and tissues. QRT-PCR showed higher RUNX3 mRNA expression in microdissected borderline and malignant ovarian tumor tissues compared with the normal ovarian surface epithelial cells (HOSE) (P=0.006 and P=0.023). Forced RUNX3 expression by lentiviral gene delivery in ovarian cancer cells, SKOV3, that initially showed undetectable RUNX3 expression, resulted in increased cell viability (P=0.043). Silencing RUNX3 expression by siRNA transfection into ovarian cancer cells, OVCAR429, initially expressing high levels of endogenous RUNX3 resulted in a decrease in proliferation (P=0.021).These results suggest that RUNX3 has a role in cell proliferation and viability in ovarian cancer.
- Subjects :
- Cytoplasm
Pathology
medicine.medical_specialty
endocrine system diseases
Cell Growth Processes
Biology
Cell Line
Ovarian carcinoma
medicine
Humans
Gene silencing
Gene Silencing
RNA, Messenger
Viability assay
RNA, Small Interfering
Ovarian Neoplasms
Regulation of gene expression
Oncogene
Ovary
Obstetrics and Gynecology
Transfection
medicine.disease
Immunohistochemistry
female genital diseases and pregnancy complications
digestive system diseases
Up-Regulation
Gene Expression Regulation, Neoplastic
Core Binding Factor Alpha 3 Subunit
Oncology
Cell culture
Cancer research
Female
Ovarian cancer
Subjects
Details
- ISSN :
- 00908258
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Gynecologic Oncology
- Accession number :
- edsair.doi.dedup.....5ff2318bec86c2a14cfb2aa04cd1d501