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δ-Atracotoxins from Australian funnel-web spiders compete with scorpion α-toxin binding on both rat brain and insect sodium channels
- Source :
- FEBS Letters, FEBS Letters, 1998, 439 (3), pp.246-252. ⟨10.1016/s0014-5793(98)01378-7⟩, FEBS Letters, Wiley, 1998, 439 (3), pp.246-252. ⟨10.1016/s0014-5793(98)01378-7⟩
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- Atracotoxins are novel peptide toxins from the venom of Australian funnel-web spiders that slow sodium current inactivation in a similar manner to scorpion alpha-toxins. To analyse their interaction with known sodium channel neurotoxin receptor sites we determined their effect on scorpion toxin, batrachotoxin and saxitoxin binding. Nanomolar concentrations of delta-atracotoxin-Hv1 and delta-atracotoxin-Ar1 completely inhibited the binding of the scorpion alpha-toxin AaH II to rat brain synaptosomes as well as the binding of LqhalphaIT, a scorpion alpha-toxin highly active on insects, to cockroach neuronal membranes. Moreover, delta-atracotoxin-Hv1 cooperatively enhanced batrachotoxin binding to rat brain synaptosomes in an analogous fashion to scorpion alpha-toxins. Thus the delta-atracotoxins represent a new class of toxins which bind to both mammalian and insect sodium channels at sites similar to, or partially overlapping with, the receptor binding sites of scorpion alpha-toxins.
- Subjects :
- Male
Insecta
[SDV]Life Sciences [q-bio]
Spider Venoms
δ-Atracotoxin
Cockroaches
Venom
Reptilian Proteins
Biochemistry
Sodium Channels
Iodine Radioisotopes
Mice
chemistry.chemical_compound
Structural Biology
Neurotoxin
Batrachotoxins
Chromatography, High Pressure Liquid
ComputingMilieux_MISCELLANEOUS
0303 health sciences
Funnel-web spider toxin
Scorpion toxin
biology
Sodium channel
030302 biochemistry & molecular biology
Brain
Spiders
Cockroach neuronal membrane
Biological Assay
Female
animal structures
Molecular Sequence Data
Neurotoxins
Biophysics
Scorpion
Scorpion Venoms
Binding, Competitive
complex mixtures
Scorpions
03 medical and health sciences
Rat brain synaptosome
biology.animal
Genetics
Animals
Amino Acid Sequence
Rats, Wistar
Molecular Biology
030304 developmental biology
Saxitoxin
HEPES
Chromatography
Sequence Homology, Amino Acid
Cell Membrane
Cell Biology
Rats
Mice, Inbred C57BL
chemistry
Batrachotoxin
Synaptosomes
Subjects
Details
- ISSN :
- 00145793 and 18733468
- Volume :
- 439
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....5fecd1ec4dc2da290fca77b4b8c51901