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Comparison of the expression and toxicity of AAV2/9 carrying the human A53T α-synuclein gene in presence or absence of WPRE
- Source :
- Heliyon, Heliyon, Elsevier 2021, 7 (2), pp.e06302. ⟨10.1016/j.heliyon.2021.e06302⟩, Heliyon, 2021, 7 (2), pp.e06302. ⟨10.1016/j.heliyon.2021.e06302⟩, Heliyon, Vol 7, Iss 2, Pp e06302-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element (WPRE) is thought to enhance transgene expression of target genes delivered by adeno-associated viral (AAV) vectors. This study assessed the protein expression of α-synuclein, phosphorylated α-synuclein at Serine 129, extent of nigrostriatal degeneration as well as subsequent behavioral deficits induced by unilateral intranigral stereotactic injection in male adult C57BL/6J mice of an AAV2/9 expressing A53T human α-synuclein under the control of the synapsin promoter in presence or absence of the WPRE. The presence of WPRE enabled to achieve greater nigrostriatal degeneration and synucleinopathy which was concomitant with worsened forelimb use asymmetry. This work refines a mouse Parkinson's disease model in which anatomo-pathology is related to behavioral deficits.<br />Parkinson's disease; Synucleinopathy; Woodchuck hepatitis virus post-transcriptional regulatory element; Mouse.
- Subjects :
- 0301 basic medicine
Parkinson's disease
Mouse
[SDV]Life Sciences [q-bio]
viruses
Transgene
Synucleinopathy
03 medical and health sciences
0302 clinical medicine
medicine
lcsh:Social sciences (General)
lcsh:Science (General)
Gene
Multidisciplinary
biology
Woodchuck hepatitis virus
Synapsin
biology.organism_classification
medicine.disease
3. Good health
Cell biology
[SDV] Life Sciences [q-bio]
Woodchuck hepatitis virus post-transcriptional regulatory element
030104 developmental biology
medicine.anatomical_structure
Stereotactic injection
Phosphorylation
lcsh:H1-99
Forelimb
030217 neurology & neurosurgery
lcsh:Q1-390
Research Article
Subjects
Details
- ISSN :
- 24058440
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Heliyon
- Accession number :
- edsair.doi.dedup.....5fe9a9fef00320229ea6419c3668826e