Back to Search
Start Over
Tuning Physicochemical Properties of a Macroporous Polysaccharide-Based Scaffold for 3D Neuronal Culture
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, 2021, 22 (23), pp.12726. ⟨10.3390/ijms222312726⟩, International Journal of Molecular Sciences, Vol 22, Iss 12726, p 12726 (2021), International Journal of Molecular Sciences, MDPI, 2021, 22 (23), pp.12726. ⟨10.3390/ijms222312726⟩, International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12726
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- Central nervous system (CNS) lesions are a leading cause of death and disability worldwide. Three-dimensional neural cultures in biomaterials offer more physiologically relevant models for disease studies, toxicity screenings or in vivo transplantations. Herein, we describe the development and use of pullulan/dextran polysaccharide-based scaffolds for 3D neuronal culture. We first assessed scaffolding properties upon variation of the concentration (1%, 1.5%, 3% w/w) of the cross-linking agent, sodium trimetaphosphate (STMP). The lower STMP concentration (1%) allowed us to generate scaffolds with higher porosity (59.9 ± 4.6%), faster degradation rate (5.11 ± 0.14 mg/min) and lower elastic modulus (384 ± 26 Pa) compared with 3% STMP scaffolds (47 ± 2.1%, 1.39 ± 0.03 mg/min, 916 ± 44 Pa, respectively). Using primary cultures of embryonic neurons from PGKCre, Rosa26tdTomato embryos, we observed that in 3D culture, embryonic neurons remained in aggregates within the scaffolds and did not attach, spread or differentiate. To enhance neuronal adhesion and neurite outgrowth, we then functionalized the 1% STMP scaffolds with laminin. We found that treatment of the scaffold with a 100 μg/mL solution of laminin, combined with a subsequent freeze-drying step, created a laminin mesh network that significantly enhanced embryonic neuron adhesion, neurite outgrowth and survival. Such scaffold therefore constitutes a promising neuron-compatible and biodegradable biomaterial.
- Subjects :
- [SDV]Life Sciences [q-bio]
Sodium trimetaphosphate
Biocompatible Materials
porous scaffold
polysaccharide
pullulan–dextran
embryonic neurons
Mice
chemistry.chemical_compound
0302 clinical medicine
Laminin
pullulan-dextran
Biology (General)
Spectroscopy
Neurons
0303 health sciences
Tissue Scaffolds
biology
Biomaterial
General Medicine
Adhesion
Computer Science Applications
[SDV] Life Sciences [q-bio]
Chemistry
Dextran
Mice, Inbred DBA
Porosity
Neurite
Cell Survival
QH301-705.5
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Polysaccharides
In vivo
Cell Adhesion
Animals
Cell Culture Techniques, Three Dimensional
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
030304 developmental biology
Tissue Engineering
Organic Chemistry
Pullulan
Embryo, Mammalian
Mice, Inbred C57BL
chemistry
Biophysics
biology.protein
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, 2021, 22 (23), pp.12726. ⟨10.3390/ijms222312726⟩, International Journal of Molecular Sciences, Vol 22, Iss 12726, p 12726 (2021), International Journal of Molecular Sciences, MDPI, 2021, 22 (23), pp.12726. ⟨10.3390/ijms222312726⟩, International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12726
- Accession number :
- edsair.doi.dedup.....5fc5515f86d8425100ff08a7c609767e
- Full Text :
- https://doi.org/10.3390/ijms222312726⟩