Back to Search
Start Over
Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica
- Source :
- Applied and environmental microbiology. 82(4)
- Publication Year :
- 2015
-
Abstract
- The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism of Y. lipolytica for using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and elucidated the native sugar metabolism in Y. lipolytica for cell growth on xylose and cellobiose as well as their mixtures with glucose through comprehensive metabolic and transcriptomic analyses. We identified 7 putative glucose-specific transporters, 16 putative xylose-specific transporters, and 4 putative cellobiose-specific transporters that are transcriptionally upregulated for growth on respective single sugars. Y. lipolytica is capable of using xylose as a carbon source, but xylose dehydrogenase is the key bottleneck of xylose assimilation and is transcriptionally repressed by glucose. Y. lipolytica has a set of 5 extracellular and 6 intracellular β-glucosidases and is capable of assimilating cellobiose via extra- and intracellular mechanisms, the latter being dominant for growth on cellobiose as a sole carbon source. Strikingly, Y. lipolytica exhibited enhanced sugar utilization for growth in mixed sugars, with strong carbon catabolite activation for growth on the mixture of xylose and cellobiose and with mild carbon catabolite repression of glucose on xylose and cellobiose. The results of this study shed light on fundamental understanding of the complex native sugar metabolism of Y. lipolytica and will help guide inverse metabolic engineering of Y. lipolytica for enhanced conversion of biomass-derived fermentable sugars to chemicals and fuels.
- Subjects :
- 0301 basic medicine
Cellobiose
Catabolite repression
Yarrowia
Xylose
7. Clean energy
Applied Microbiology and Biotechnology
Metabolic engineering
03 medical and health sciences
chemistry.chemical_compound
Xylose metabolism
2. Zero hunger
Ecology
biology
Gene Expression Profiling
biology.organism_classification
Yeast
Carbon
030104 developmental biology
Glucose
chemistry
Biochemistry
Metabolome
Fermentation
Erratum
Metabolic Networks and Pathways
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10985336
- Volume :
- 82
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Applied and environmental microbiology
- Accession number :
- edsair.doi.dedup.....5fbf9354016e35425d81d93bc5a46103