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Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast Purification and biological characterization
- Source :
- FEBS Letters. (2):231-236
- Publisher :
- Published by Elsevier B.V.
-
Abstract
- The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A 2 , Ca 2+ -dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.
- Subjects :
- Recombinant protein
Annexins
Neutrophils
Saccharomyces cerevisiae
Genetic Vectors
Guinea Pigs
Biophysics
Biology
Lipocortin
Biochemistry
Protein kinase
Structural Biology
Complementary DNA
HSPA2
Genetics
Animals
Cloning, Molecular
Protein kinase A
Molecular Biology
Expression vector
Binding protein
Calcium-Binding Proteins
Neutrophil
Cell Biology
DNA
biology.organism_classification
Molecular biology
Yeast
Recombinant Proteins
Molecular Weight
Phospholipases
Tyrosine kinase
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....5f82ec1f4e33ad0d2f472599ed574ce1
- Full Text :
- https://doi.org/10.1016/0014-5793(89)81097-X