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Characterization of the mouse white adipose tissue redox environment and associations with perinatal environmental exposures to bisphenol A and high-fat diets
- Publication Year :
- 2019
-
Abstract
- White adipose tissue (WAT) plays an important role in obesity pathophysiology. Redox signaling underlies several aspects of WAT physiology; however, the thiol redox environment of WAT has not yet been fully characterized. Dietary and endocrine disrupting chemical (EDC) exposures during development can transiently impact the cellular redox environment, but it is unknown whether these exposures can reprogram the WAT thiol redox environment. To characterize the WAT thiol redox environment, we took a descriptive approach and measured thiol redox parameters using high-performance liquid chromatography in mouse mesenteric (mWAT), gonadal (gWAT) and subinguinal (sWAT) depots. Cysteine (CYSS:CYS) and glutathione (GSSG:GSH) redox potentials (Eh) were more oxidizing in gWAT and sWAT than mWAT. Increased body weight, relative WAT weight and age were associated with oxidizing GSSG:GSH Eh in mWAT in a sex-specific manner. Body weight and relative WAT weight were also positively associated with GSSG:GSH Eh in sWAT. We carried out a second mouse study with perinatal exposures to bisphenol A (BPA) and Mediterranean and Western high-fat diets (HFDs) to determine whether early-life chemical and dietary factors have long-lasting impacts on mWAT redox parameters. Mice exposed to Mediterranean HFD or BPA had more oxidizing GSSG:GSH mWAT Eh than controls, with more pronounced differences in females. These findings suggest an important role for the thiol redox environment in WAT physiology. Observed sex-specific and depot-specific differences in thiol redox parameters are consistent with known WAT physiology. Lastly, mWAT GSSG:GSH Eh may be reprogrammed by developmental exposure to HFDs and EDCs, which may have implications for obesity risk.
- Subjects :
- 0301 basic medicine
Male
Bisphenol A
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
White adipose tissue
Diet, Mediterranean
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Pregnancy
Longitudinal Studies
Chromatography, High Pressure Liquid
Adiposity
chemistry.chemical_classification
Nutrition and Dietetics
food and beverages
Glutathione
Prenatal Exposure Delayed Effects
Female
Oxidation-Reduction
hormones, hormone substitutes, and hormone antagonists
medicine.medical_specialty
Adipose Tissue, White
030209 endocrinology & metabolism
Diet, High-Fat
Redox
Article
03 medical and health sciences
Phenols
Internal medicine
medicine
Animals
Cysteine
Obesity
Benzhydryl Compounds
Molecular Biology
Reactive oxygen species
Mouse White Adipose Tissue
Body Weight
nutritional and metabolic diseases
High fat diet
Environmental Exposure
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
chemistry
Diet, Western
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....5f814af27d31c69fd3eb610372f99b69