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Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives
- Source :
- Biotechnology Letters. 37:1655-1661
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- To examine nitrilase-mediated hydrolysis of nitriles to produce optically pure α-hydroxycarboxylic acids.A novel nitrilase, GPnor51, from Luminiphilus syltensis NOR5-1B was discovered by genomic data mining. It could hydrolyze racemic o-chloromandelonitrile to (R)-o-chloromandelic acid with high enantioselectivity (ee 98.2 %). GPnor51 was overexpressed in Escherichia coli BL21 (DE3), purified, and its catalytic properties studied. GPnor51 had a broad substrate acceptance toward various nitriles with structure diversity. It was an arylacetonitrilase that uses arylacetonitriles as optimal substrates. The V max and K m of GPnor51 towards o-chloromandelonitrile were 1.9 μmol min(-1) mg(-1) protein and 0.38 mM, respectively. GPnor51 also demonstrated high enantioselectivity toward mandelonitrile and other substituted mandelonitrile.This enzyme has a great potential for commercial production of optically pure (R)-mandelic acid and its derivatives.
- Subjects :
- genetic structures
Bioengineering
Applied Microbiology and Biotechnology
Luminiphilus syltensis
Nitrilase
Substrate Specificity
chemistry.chemical_compound
Hydrolysis
Aminohydrolases
Nitriles
Escherichia coli
Data Mining
Organic chemistry
Arylacetonitrilase
Cloning, Molecular
Cloning
chemistry.chemical_classification
Computational Biology
General Medicine
Mandelic acid
Recombinant Proteins
eye diseases
Kinetics
Enzyme
chemistry
Biocatalysis
Mandelic Acids
Gammaproteobacteria
Genome, Bacterial
Biotechnology
Subjects
Details
- ISSN :
- 15736776 and 01415492
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....5f769d91840b9d308b61164300ec5f94