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A Commensal Strain of Staphylococcus epidermidis Overexpresses Membrane Proteins Associated with Pathogenesis When Grown in Biofilms
- Source :
- The Journal of Membrane Biology. 248:431-442
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Staphylococcus epidermidis has emerged as one of the major nosocomial pathogens associated with infections of implanted medical devices. The most important factor in the pathogenesis of these infections is the formation of bacterial biofilms. Bacteria grown in biofilms are more resistant to antibiotics and to the immune defence system than planktonic bacteria. In these infections, the antimicrobial therapy usually fails and the removal of the biofilm-coated implanted device is the only effective solution. In this study, three proteomic approaches were performed to investigate membrane proteins associated to biofilm formation: (i) sample fractionation by gel electrophoresis, followed by isotopic labelling and LC-MS/MS analysis, (ii) in-solution sample preparation, followed by isotopic labelling and LC-MS/MS analysis and (iii) in-solution sample preparation and label-free LC-MS/MS analysis. We found that the commensal strain S. epidermidis CECT 231 grown in biofilms expressed higher levels of five membrane and membrane-associated proteins involved in pathogenesis: accumulation-associated protein, staphylococcal secretory antigen, signal transduction protein TRAP, ribonuclease Y and phenol soluble modulin beta 1 when compared with bacteria grown under planktonic conditions. These results indicate that a commensal strain can acquire a pathogenic phenotype depending on the mode of growth.
- Subjects :
- Virulence Factors
Physiology
medicine.drug_class
Antibiotics
Biophysics
Gene Expression
Virulence
Microbiology
Bacterial Proteins
Tandem Mass Spectrometry
Staphylococcus epidermidis
medicine
Gel electrophoresis
biology
Biofilm
Gene Expression Regulation, Bacterial
Cell Biology
Antimicrobial
biology.organism_classification
Up-Regulation
Membrane protein
Biofilms
Bacteria
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 14321424 and 00222631
- Volume :
- 248
- Database :
- OpenAIRE
- Journal :
- The Journal of Membrane Biology
- Accession number :
- edsair.doi.dedup.....5f6dca36166e39e8701b793195785cfc