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Meniscal Tissue Engineering Using Aligned Collagen Fibrous Scaffolds: Comparison of Different Human Cell Sources
- Source :
- Tissue engineering. Part A, vol 24, iss 1-2
- Publication Year :
- 2018
- Publisher :
- Mary Ann Liebert Inc, 2018.
-
Abstract
- Hydrogel and electrospun scaffold materials support cell attachment and neotissue development and can be tuned to structurally and mechanically resemble native extracellular matrix by altering either electrospun fiber or hydrogel properties. In this study, we examined meniscus tissue generation from different human cell sources including meniscus cells derived from vascular and avascular regions, human bone marrow-derived mesenchymal stem cells, synovial cells, and cells from the infrapatellar fat pad (IPFP). All cells were seeded onto aligned electrospun collagen type I scaffolds and were optionally encapsulated in a tricomponent hydrogel. Single or multilayered constructs were generated and cultivated in defined medium with selected growth factors for 2 weeks. Cell viability, cell morphology, and gene-expression profiles were monitored using confocal microscopy, scanning electron microscopy, and quantitative polymerase chain reaction (qPCR), respectively. Multilayered constructs were examined with histology, immunohistochemistry, qPCR, and for tensile mechanical properties. For all cell types, TGFβ1 and TGFβ3 treatment increased COL1A1, COMP, Tenascin C (TNC), and Scleraxis (SCX) gene expression and deposition of collagen type I protein. IPFP cells generated meniscus-like tissues with higher meniscogenic gene expression, mechanical properties, and better cell distribution compared to other cell types studied. We show proof of concept that electrospun collagen scaffolds support neotissue formation and IPFP cells have potential for use in cell-based meniscus regeneration strategies.
- Subjects :
- Male
0301 basic medicine
Cell Count
02 engineering and technology
Regenerative Medicine
Cell morphology
Biochemistry
Extracellular matrix
meniscus
Tissue engineering
Stem Cell Research - Nonembryonic - Human
Scanning
Cells, Cultured
Microscopy
Cultured
Tissue Scaffolds
Chemistry
021001 nanoscience & nanotechnology
Female
Stem Cell Research - Nonembryonic - Non-Human
Collagen
Development of treatments and therapeutic interventions
0210 nano-technology
Adult stem cell
Adult
Cell type
Cells
Biomedical Engineering
Bioengineering
adult stem cells
Electron
Biomaterials
03 medical and health sciences
Humans
Meniscus
biomimetic materials
Tissue Engineering
5.2 Cellular and gene therapies
Mesenchymal stem cell
Scleraxis
Original Articles
Materials Engineering
Stem Cell Research
030104 developmental biology
Synovial Cell
Musculoskeletal
Microscopy, Electron, Scanning
Biophysics
Biochemistry and Cell Biology
Biomedical engineering
Subjects
Details
- ISSN :
- 1937335X and 19373341
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering Part A
- Accession number :
- edsair.doi.dedup.....5f5c7c486d4bbccc97560176f28b6b65