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Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop
- Source :
- EMBO Journal, EMBO Journal, EMBO Press, 2002, 21 (7), pp.1811-1820. ⟨10.1093/emboj/21.7.1811⟩
- Publication Year :
- 2002
- Publisher :
- HAL CCSD, 2002.
-
Abstract
- International audience; The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2′‐O‐rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that Trm7p methylates the 2′‐O‐ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro. In a trm7Δ strain, which is viable but grows slowly, translation is impaired, thus indicating that these tRNA modifications could be important for translation efficiency. We discuss the emergence of a family of three 2′‐O‐RNA methyltransferases in Eukaryota and one in Prokaryota from a common ancestor. We propose that each eukaryotic enzyme is located in a different cell compartment, in which it would methylate a different RNA that can adopt a very similar secondary structure.
- Subjects :
- S-Adenosylmethionine
Methyltransferase
Ribose
[SDV]Life Sciences [q-bio]
Cell Cycle Proteins
RNA-binding protein
RNA, Transfer
Maturation
Protein biosynthesis
Genetics
tRNA Methyltransferases
0303 health sciences
General Neuroscience
030302 biochemistry & molecular biology
Translation (biology)
Three‐Dimensional Structure
Eukaryotic Cells
Biochemistry
Transfer RNA
Saccharomyces cerevisiae Proteins
RNA Methyltransferase
Recombinant Fusion Proteins
Molecular Sequence Data
Saccharomyces cerevisiae
Modification
Biology
Catalysis
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Anticodon
Escherichia coli
Animals
Humans
Amino Acid Sequence
RNA, Messenger
Molecular Biology
030304 developmental biology
Base Sequence
Sequence Homology, Amino Acid
General Immunology and Microbiology
RNA
RNA, Fungal
Methyltransferases
biology.organism_classification
Protein Structure, Tertiary
TRNA Methyltransferases
RNA Binding Protein
Mutagenesis
Protein Biosynthesis
Nucleic Acid Conformation
Subjects
Details
- Language :
- English
- ISSN :
- 02614189 and 14602075
- Database :
- OpenAIRE
- Journal :
- EMBO Journal, EMBO Journal, EMBO Press, 2002, 21 (7), pp.1811-1820. ⟨10.1093/emboj/21.7.1811⟩
- Accession number :
- edsair.doi.dedup.....5f57efc3fb20ff591878e77e3684128c