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Lowry protein assay using an automatic microtiter plate spectrophotometer
- Source :
- Analytical biochemistry. 153(2)
- Publication Year :
- 1986
-
Abstract
- The method of protein determination reported by Lowry et al. (1951, J. Biol. Chem. 193, 265-275) has been adapted for use with 96-well microtiter plates and an automatic microplate spectrophotometer. The spectrophotometer has been interfaced with a computer which plots the standard curve and calculates the protein content of each sample. The adapted method offers advantages over previously reported methods in that it is more rapid and uses a smaller sample volume (100 microliters) for samples containing 3-300 micrograms/ml (0.3-30 micrograms/assay) of protein. The method of Bensadoun and Weinstein (1976, Anal. Biochem. 70, 241-252) for precipitating microgram amounts of protein away from substances which interfere with the Lowry assay has also been adapted to this microplate procedure. These techniques should be particularly useful for laboratories where large numbers of samples containing a wide range of protein concentrations are assayed.
- Subjects :
- Chromatography
Chemistry
Computers
Biophysics
Proteins
Serum Albumin, Bovine
Cell Biology
Reference Standards
Biochemistry
Standard curve
Protein content
Sample volume
Microtiter plate
Spectrophotometry
Lowry protein assay
Bicinchoninic acid assay
Animals
Cattle
Molecular Biology
Reference standards
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 153
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Analytical biochemistry
- Accession number :
- edsair.doi.dedup.....5f54ef5e5a967ee04a573bbc99138148