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Cloning, expression and evaluation of a recombinant sub-unit vaccine against Clostridium botulinum type F toxin
- Source :
- Vaccine. 19:288-297
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- A synthetic gene encoding the Hc (binding) domain of Clostridium botulinum neurotoxin F (FHc) was expressed in Escherichia coli fused to maltose binding protein (MBP). The purified MBP–FHc and FHc isolated after removal of MBP were evaluated in mice for their ability to protect against toxin challenge. Balb/c mice developed a protective immune response following administration of either protein via the intraperitoneal or intramuscular routes. A comparison of antibody titres and protection following single and multiple vaccinations and the effects of dosage are shown. The long term protection afforded by the vaccines was also investigated. Ten months following vaccination mice were still protected when challenged with 10 4 MLD 50 doses of botulinum toxin F.
- Subjects :
- Clostridium botulinum type F
Botulinum Toxins
Monosaccharide Transport Proteins
Recombinant Fusion Proteins
medicine.disease_cause
Maltose-Binding Proteins
Microbiology
Mice
Maltose-binding protein
Escherichia coli
medicine
Animals
Neurotoxin
Cloning, Molecular
Mice, Inbred BALB C
Vaccines, Synthetic
General Veterinary
General Immunology and Microbiology
biology
Toxin
Escherichia coli Proteins
Immunogenicity
Vaccination
Public Health, Environmental and Occupational Health
Antibodies, Bacterial
Virology
Infectious Diseases
Bacterial Vaccines
Humoral immunity
biology.protein
Molecular Medicine
Clostridium botulinum
ATP-Binding Cassette Transporters
Female
Antibody
Carrier Proteins
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....5f5013b512146d5009d8902da1dc8c0e