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Secondary Metabolite Profiling of Curcuma Species Grown at Different Locations Using GC/TOF and UPLC/Q-TOF MS

Authors :
Byeong Cheol Moon
Ho Kyoung Kim
Jeoung-Hwa Shin
Geum-Sook Hwang
Youngae Jung
Do Hyun Ryu
Jueun Lee
Source :
Molecules; Volume 19; Issue 7; Pages: 9535-9551, Molecules, Vol 19, Iss 7, Pp 9535-9551 (2014), Molecules
Publication Year :
2014
Publisher :
Multidisciplinary Digital Publishing Institute, 2014.

Abstract

Curcuma, a genus of rhizomatous herbaceous species, has been used as a spice, traditional medicine, and natural dye. In this study, the metabolite profile of Curcuma extracts was determined using gas chromatography-time of flight mass spectrometry (GC/TOF MS) and ultrahigh-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) to characterize differences between Curcuma aromatica and Curcuma longa grown on the Jeju-do or Jin-do islands, South Korea. Previous studies have performed primary metabolite profiling of Curcuma species grown in different regions using NMR-based metabolomics. This study focused on profiling of secondary metabolites from the hexane extract of Curcuma species. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the C. aromatica and C. longa metabolite profiles, whereas geographical location had little effect. A t-test was performed to identify statistically significant metabolites, such as terpenoids. Additionally, targeted profiling using UPLC/Q-TOF MS showed that the concentration of curcuminoids differed depending on the plant origin. Based on these results, a combination of GC- and LC-MS allowed us to analyze curcuminoids and terpenoids, the typical bioactive compounds of Curcuma, which can be used to discriminate Curcuma samples according to species or geographical origin.

Details

Language :
English
ISSN :
14203049
Database :
OpenAIRE
Journal :
Molecules; Volume 19; Issue 7; Pages: 9535-9551
Accession number :
edsair.doi.dedup.....5f4c9e48838961554900d8f3f0f53967
Full Text :
https://doi.org/10.3390/molecules19079535