Back to Search
Start Over
Secondary Metabolite Profiling of Curcuma Species Grown at Different Locations Using GC/TOF and UPLC/Q-TOF MS
- Source :
- Molecules; Volume 19; Issue 7; Pages: 9535-9551, Molecules, Vol 19, Iss 7, Pp 9535-9551 (2014), Molecules
- Publication Year :
- 2014
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2014.
-
Abstract
- Curcuma, a genus of rhizomatous herbaceous species, has been used as a spice, traditional medicine, and natural dye. In this study, the metabolite profile of Curcuma extracts was determined using gas chromatography-time of flight mass spectrometry (GC/TOF MS) and ultrahigh-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) to characterize differences between Curcuma aromatica and Curcuma longa grown on the Jeju-do or Jin-do islands, South Korea. Previous studies have performed primary metabolite profiling of Curcuma species grown in different regions using NMR-based metabolomics. This study focused on profiling of secondary metabolites from the hexane extract of Curcuma species. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the C. aromatica and C. longa metabolite profiles, whereas geographical location had little effect. A t-test was performed to identify statistically significant metabolites, such as terpenoids. Additionally, targeted profiling using UPLC/Q-TOF MS showed that the concentration of curcuminoids differed depending on the plant origin. Based on these results, a combination of GC- and LC-MS allowed us to analyze curcuminoids and terpenoids, the typical bioactive compounds of Curcuma, which can be used to discriminate Curcuma samples according to species or geographical origin.
- Subjects :
- Curcumin
Metabolite
Pharmaceutical Science
Curcuma aromatica
Zngiberaceae
Secondary metabolite
High-performance liquid chromatography
Gas Chromatography-Mass Spectrometry
Article
Analytical Chemistry
lcsh:QD241-441
chemistry.chemical_compound
Curcuma longa
metabolite profiling
secondary metabolites
GC/TOF MS
UPLC/Q-TOF MS
Curcuma
Metabolomics
lcsh:Organic chemistry
Diarylheptanoids
Republic of Korea
Drug Discovery
medicine
Physical and Theoretical Chemistry
Chromatography, High Pressure Liquid
Chromatography
biology
Plant Extracts
Chemistry
Organic Chemistry
Primary metabolite
biology.organism_classification
Terpenoid
Chemistry (miscellaneous)
Molecular Medicine
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules; Volume 19; Issue 7; Pages: 9535-9551
- Accession number :
- edsair.doi.dedup.....5f4c9e48838961554900d8f3f0f53967
- Full Text :
- https://doi.org/10.3390/molecules19079535