Additional file 2: of Stemness marker ALDH1A1 promotes tumor angiogenesis via retinoic acid/HIF-1Îą/VEGF signalling in MCF-7 breast cancer cells

Figure S1. Loss-of function and gain-of function validation to study ALDH1A1 in MCF-7 cells. a. RT-PCR in breast tumor cells (Src and ALDH1A1KD, clones shA and shB) cultured in 10 % FBS for 48 h. ***p < 0.001 vs Scr cells. b. Western blot analysis of breast tumor cells (Src and ALDH1A1KD, clones shA and shB) cultured in 10 % FBS for 48 h. c. RT-PCR analysis of MCF-7 (Src, ALDH1A1KD and ALDH1A1+) cultured in 10 % FBS for 48 h. ***p < 0.001 vs MCF-7 Scr. ###p < 0.001 vs MCF-7 ALDH1A1KD (n = 3). d. Western blot analysis of MCF-7 (Src, ALDH1A1KD and ALDH1A1+) cultured in 10 % FBS for 48 h. β-actin was used as loading control. Gel shown is representative of three experiments with similar results. e. Enzymatic activity in MCF-7 ALDH1A1KD and ALDH1A1+ evaluated by NADH production. Data are reports as in Figure 1. ***p < 0.001 vs MCF-7 Scr. ###p < 0.001 vs MCF-7 ALDH1A1KD. (PDF 372 kb)