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Association of bovine papillomavirus type 2 (BPV-2) and urinary bladder tumours in cattle from the Azores archipelago
- Source :
- Research in Veterinary Science. 90:526-529
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Urinary bladder tumours in cattle are caused by chronic ingestion of bracken fern and BPV-1/2 infection. The objective of the present study was to assess if BPV-2 was present in urinary bladder lesions from cattle with chronic enzootic haematuria (CEH) from the Azores archipelago (Portugal), in order to gain further information regarding the epidemiologic distribution of this virus. Samples were analysed using PCR specific primers for BPV-2 DNA and an immunohistochemistry for BPV E5 oncoprotein detection. We found a 28% incidence rate of BPV-2 DNA in different types of tumours and cystitis cases (13 out of 46 samples). Tested positive samples for PCR were also positive for the viral E5 oncoprotein; protein immunolabeling was mainly detected within the cytoplasm of urothelial cells, displaying a juxtanuclear distribution. This is the first report of BPV-2 detection in urinary bladder tumours associated with CEH in cattle from the Azores archipelago.
- Subjects :
- Pathology
medicine.medical_specialty
viruses
Urinary Bladder
Cattle Diseases
Biology
Polymerase Chain Reaction
Virus
Pathology and Forensic Medicine
Immunolabeling
medicine
Animals
Azores archipelago
Azores
Bovine papillomavirus 1
Hematuria
Bovine papillomavirus
Gynecology
Urinary bladder
General Veterinary
business.industry
Papillomavirus Infections
Chronic ingestion
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Virology
medicine.anatomical_structure
Urinary Bladder Neoplasms
Specific primers
DNA, Viral
Immunohistochemistry
Cattle
Female
business
Subjects
Details
- ISSN :
- 00345288
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- Research in Veterinary Science
- Accession number :
- edsair.doi.dedup.....5f365d772343f11dcc3f350baab3e1e0