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A nascent micronuclear pseudogene in the ciliate Euplotes crassus
- Source :
- Nucleic Acids Research. 24:3195-3200
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- The macronuclear genome of Euplotes crassus contains two different genes, EFA1 and EFA2, encoding EF-1alpha proteins. They are derived from micronuclear precursors in the course of a sexual process termed conjugation. We have found that two apparent micronuclear precursors exist for EFA1. They differ in their potential coding sequences and in the internal sequences interrupting the genes, which are normally removed during the processing from micronuclear into macronuclear genes. One of these genes is not processed into a macronuclear gene and has accumulated C-->T transitions in a limited section of the coding region. The gene obviously constitutes a recent duplication which has lost its ability to be processed into a macronuclear gene and has therefore become a micronuclear pseudogene. The true EFA1 precursor harbours a novel type of internal sequence in addition to a classical AT-rich IES. As usual, only one micronuclear EFA2 precursor gene was found. Its coding sequence is interrupted by a 79 bp TeIIES.
- Subjects :
- Pseudogene
Genes, Protozoan
Molecular Sequence Data
Euplotes
Mutagenesis (molecular biology technique)
Biology
Polymerase Chain Reaction
Genome
Chromosomes
chemistry.chemical_compound
Peptide Elongation Factor 1
Gene duplication
Genetics
Animals
Coding region
Amino Acid Sequence
Gene
Peptide sequence
Polymorphism, Single-Stranded Conformational
Sequence Deletion
Cell Nucleus
Base Sequence
Sequence Analysis, DNA
DNA, Protozoan
Peptide Elongation Factors
chemistry
Mutagenesis
Micronucleus, Germline
Pseudogenes
DNA
Research Article
Subjects
Details
- ISSN :
- 13624962
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....5f3539b857e2515cdc052abf543c18cc