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Differential Staining of Calcified Tissues in Plastic Embedded Microtome Sections by A Modification of Movat's Pentachrome Stain
- Source :
- Stain Technology. 52:331-337
- Publication Year :
- 1977
- Publisher :
- Informa UK Limited, 1977.
-
Abstract
- Movat's pentachrome I stain has been adapted and modified as a stain for undecalcified bone sections. After embedding in methyl methacrylate, this procedure yields consistently good results, with an excellent and colorful contrast between mineralized and unmineralized compartments of both cartilage and bone. In addition, osteoblasts, osteoclasts, and other cells and tissue components can easily be differentiated. The staining properties of the lacunar wall surrounding the osteocytes are considered to reflect various states of osteocytic activity. The method is especially useful for the study of bone growth and bone repair, and as a stain for conventional histomorphometry and computer-assisted image analysis in bone biopsies.
- Subjects :
- Pathology
medicine.medical_specialty
Bone healing
Stain
Bone and Bones
law.invention
Calcification, Physiologic
Dogs
law
Microtome
medicine
Animals
Humans
Methylmethacrylates
Bone growth
Staining and Labeling
Chemistry
Differential staining
Cartilage
Decalcification Technique
Microtomy
Anatomy
Rats
Staining
Undecalcified bone
medicine.anatomical_structure
Subjects
Details
- ISSN :
- 00389153
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Stain Technology
- Accession number :
- edsair.doi.dedup.....5f35264974ccdd1d00148cfbd79cd9b6