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Fluorescence resonance energy transfer-thermal lens spectrometry (FRET-TLS) as molecular counting of methamphetamine
- Source :
- Microchimica Acta. 188
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- A novel and sensitive approach has been presented for the determination of methamphetamine (METH) based on fluorescence resonance energy transfer-thermal lens spectrometry (FRET-TLS). Due to the affinity of fluorescein molecules to the surface of AuNPs through the electrostatic interaction and thereby caused reduction of the distance between fluorescein and AuNPs, the best way for de-excitation of excited fluorescein is FRET. The energy absorbed by fluorescein transferred to AuNPs causes enhancement of the thermal lens effect. The thermal lens of the fluorescence molecule could be enhanced through a proper acceptor. Upon the addition of methamphetamine, the fluorescein molecules are detached from the surface of AuNPs, due to the stronger adsorption of methamphetamine. As a result, the fluorescence of fluorescein recovered, and the thermal lens effect of fluorescein decreased. The mechanism of energy transfer was evaluated by two different methods including time-resolved spectroscopy and thermal lens spectrometry. Under the optimal conditions, the thermal lens signal was linearly proportional to methamphetamine concentration in the range 5 - 80 nM. The limit of detection and limit of quantitation were 1.5 nM and 4.5 nM, respectively. The detection volume and limit of molecules in the detection volume were 960 attoliter and 87 molecules, respectively. The method was successfully applied for the determination of methamphetamine in human blood plasma and urine.
- Subjects :
- Metal Nanoparticles
02 engineering and technology
Mass spectrometry
Photochemistry
01 natural sciences
Methamphetamine
Analytical Chemistry
law.invention
chemistry.chemical_compound
Limit of Detection
law
Fluorescence Resonance Energy Transfer
Humans
Fluorescein
Spectroscopy
Fluorescent Dyes
Detection limit
010401 analytical chemistry
Fluoresceins
021001 nanoscience & nanotechnology
Fluorescence
0104 chemical sciences
Lens (optics)
Förster resonance energy transfer
chemistry
Adsorption
Gold
Time-resolved spectroscopy
0210 nano-technology
Subjects
Details
- ISSN :
- 14365073 and 00263672
- Volume :
- 188
- Database :
- OpenAIRE
- Journal :
- Microchimica Acta
- Accession number :
- edsair.doi.dedup.....5f0ce0b533521bec19fa78985c9a92a1