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TBHQ alleviates pyroptosis and necroptosis in chicken alveolar epithelial cells induced by fine particulate matter from broiler houses
- Source :
- Poultry Science, Poultry Science, Vol 101, Iss 2, Pp 101593-(2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Fine particulate matter (PM2.5) from poultry houses has adverse effects on the health of animals and workers. Tert-butylhydroquinone (TBHQ), an antioxidant, is widely used in feed additives. The present study investigated the effects of TBHQ on broiler house PM2.5-induced damage in chicken primary alveolar epithelial cells (AECII) extracted from 16-day-old chicken embryos using the method of differential adhesion. AECII were exposed to PM2.5 and TBHQ alone or in combination, and then, cell membrane integrity, pyroptosis, and necroptosis were detected. Our results showed that PM2.5 from broiler houses caused cell rupture and loss of cell membrane integrity. This result was confirmed by the obvious increases in lactate dehydrogenase (LDH) release and propidium iodide (PI)-positive cells compared to the control group. In addition, the intracellular reactive oxygen species (ROS) levels and the expression levels of pyroptosis-related genes (NLRP3, IL-18, IL-1β) and necroptosis-related genes (RIPK3) were also significantly enhanced. However, TBHQ significantly inhibited intracellular ROS, improved cell viability, and reduced the release of LDH and the number of PI-positive cells compared to those in the PM2.5 group. The expression levels of pyroptosis-related genes (Caspase-1, NLRP3, IL-18, IL-1β) and necroptosis-related genes (RIPK3) were also significantly decreased in the co-treatment group. In summary, these results indicated that TBHQ can alleviate PM2.5-mediated cell pyroptosis and necroptosis in chicken AECII and provide a basis for overcoming the danger that air pollutants from broiler houses pose to the health of chickens.
- Subjects :
- Antioxidant
medicine.medical_treatment
Necroptosis
Cell
necroptosis
Chick Embryo
SF1-1100
chicken alveolar epithelial cell
chemistry.chemical_compound
Lactate dehydrogenase
NLR Family, Pyrin Domain-Containing 3 Protein
medicine
Animals
Propidium iodide
Viability assay
GENETICS AND MOLECULAR BIOLOGY
pyroptosis
Pyroptosis
General Medicine
Animal culture
Hydroquinones
Cell biology
medicine.anatomical_structure
fine particulate matter
chemistry
Alveolar Epithelial Cells
TBHQ
Particulate Matter
Animal Science and Zoology
Chickens
Intracellular
Subjects
Details
- ISSN :
- 00325791
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Poultry Science
- Accession number :
- edsair.doi.dedup.....5effaa38e0a1e41569598c82468dde66
- Full Text :
- https://doi.org/10.1016/j.psj.2021.101593