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miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 3, p e0174297 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- Purpose The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group. Methods In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors. Results Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan® assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p: < 0.00004), mir-24 (FC -7.52, p: < 0.004) and miR-142-3p (FC -5.32, p: < 0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM. Conclusion The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM.
- Subjects :
- 0301 basic medicine
genetic structures
Eye Diseases
medicine.medical_treatment
lcsh:Medicine
Vitrectomy
Exosomes
Biochemistry
Transcriptome
0302 clinical medicine
Medicine and Health Sciences
lcsh:Science
Prospective cohort study
Macular hole
Multidisciplinary
Ophthalmic Procedures
Nucleic acids
RNA extraction
Epiretinal membrane
Anatomy
Cellular Structures and Organelles
Research Article
Surgical and Invasive Medical Procedures
03 medical and health sciences
Extraction techniques
Ocular System
microRNA
medicine
Genetics
Vesicles
Non-coding RNA
MED/30 Malattie apparato visivo
Biology and life sciences
business.industry
lcsh:R
Cell Biology
medicine.disease
Molecular biology
Fibrosis
Gene regulation
Research and analysis methods
MicroRNAs
Ophthalmology
030104 developmental biology
Vitreous chamber
030221 ophthalmology & optometry
RNA
Eyes
lcsh:Q
Gene expression
business
Head
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....5eebf44904116d191ff9886578de6000