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Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system
- Source :
- Biochemical and biophysical research communications. 443(4)
- Publication Year :
- 2013
-
Abstract
- Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.
- Subjects :
- medicine.medical_specialty
Cell
Cytological Techniques
Biophysics
Biosensing Techniques
Biology
Biochemistry
Dexamethasone
Rosiglitazone
Mice
Computer Systems
Internal medicine
1-Methyl-3-isobutylxanthine
3T3-L1 Cells
medicine
Adipocytes
Electric Impedance
Animals
Insulin
Real time analysis
Molecular Biology
End point
Adipogenesis
Cell substrate
Impedance sensor
3T3-L1
Cell Differentiation
Cell Biology
Cell biology
Endocrinology
medicine.anatomical_structure
Thiazolidinediones
Single plate
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 443
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....5ed96e184214406e949eb7c7cf201e64