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Differential Gene Expression in the Meristem and during Early Fruit Growth of Pisum sativum L. Identifies Potential Targets for Breeding
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 18, Iss 2, p 428 (2017), International Journal of Molecular Sciences; Volume 18; Issue 2; Pages: 428
- Publication Year :
- 2016
-
Abstract
- For successful molecular breeding it is important to identify targets to the gene family level, and in the specific species of interest, in this case Pisum sativum L. The cytokinins have been identified as a key breeding target due to their influence on plant architecture, and on seed size and sink activity. We focused on the cytokinin biosynthetic gene family (the IPTs) and the gene family key to the destruction of cytokinins (the CKXs), as well as other gene families potentially affected by changing cytokinin levels. These included key meristem genes (WUS and BAM1) and the transporter gene families, sucrose transporters (SUTs) and amino acid permeases (AAPs). We used reverse transcription quantitative PCR (RT-qPCR) to monitor gene expression in the vegetative meristem and in pre- and post-fertilisation young pea fruits. PsWUS expression was specific to the shoot apical meristem while PsBAM1 was highly expressed in the shoot apical meristem (SAM) but was also expressed at a low level in the young fruit. Differential expression was shown between genes and within gene families for IPT, CKX, SUT, and AAP. PsCKX7 showed strong gene family member-specific expression in the SAM, and was also expressed in young pea fruits. We suggest that PsCKX7 is a potential target for downregulation via molecular breeding or gene editing.
- Subjects :
- 0106 biological sciences
0301 basic medicine
process pea
cytokinin
isopentenyl transferase
cytokinin oxidase/dehydrogenase
sucrose transporter
amino acid permease
WUSCHEL
BAM1
field pea
gene editing
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Gene Expression Regulation, Plant
Gene expression
Cluster Analysis
lcsh:QH301-705.5
Spectroscopy
Phylogeny
Molecular breeding
Genetics
biology
food and beverages
General Medicine
Computer Science Applications
ABC model of flower development
Cytokinin
Plant Shoots
Meristem
Catalysis
Article
Pisum
Inorganic Chemistry
03 medical and health sciences
Gene family
Physical and Theoretical Chemistry
Molecular Biology
Gene
Gene Expression Profiling
Organic Chemistry
fungi
Peas
biology.organism_classification
Plant Breeding
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Fruit
Transcriptome
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....5ecbac41b5a90721de3a795484d60eda