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Isolation and characterization of microsatellite loci in Symbiodinium B1/B184, the dinoflagellate symbiont of the Caribbean sea fan coral, Gorgonia ventalina

Authors :
Jason P. Andras
Nathan L. Kirk
C. Drew Harvell
Mary Alice Coffroth
Source :
Molecular ecology resources. 9(3)
Publication Year :
2011

Abstract

Here we report primers targeting 10 microsatellite loci of dinoflagellates in the genus Symbiodinium (clade B1/B184) symbiotic with the Caribbean sea fan coral, Gorgonia ventalina. Primers were tested on 12 Symbiodinium B1/B184 cultures, as well as 40 genomic DNA extracts of G. ventalina tissue samples. All loci were polymorphic with allelic richness ranging from 4-16. Gene diversity ranged from 0.15 to 0.91. These primers provide powerful tools for examining the fine-scale population structure and dynamics of Symbiodinium within a single host species.

Details

ISSN :
1755098X
Volume :
9
Issue :
3
Database :
OpenAIRE
Journal :
Molecular ecology resources
Accession number :
edsair.doi.dedup.....5eca71ad272cda2d0f4a3f66d32bc012