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Sequence Polymorphism in Xenobiotic Metabolising Genes in Iraqi Colorectal Cancer Patients
- Source :
- Asian Pacific Journal of Cancer Prevention : APJCP
- Publication Year :
- 2021
- Publisher :
- West Asia Organization for Cancer Prevention, 2021.
-
Abstract
- Objectives: Colorectal cancer (CRC) is the third most prevalent malignant neoplasm. Genetic variations in the xenobiotic metabolising cytochrome enzymes. Family 1 Subfamily A Member 1 (CYP1A1) and Family 1 Subfamily B Member 1 (CYP1B1) might play a role in cancer pathogenesis and prognosis. The aim of this work is to determine the frequency of Single Nucleotide Polymorphisms (SNPs) in CYP1A1 (rs1048943, Ile462VaI and rs4646903/MSP1) and CYP1B1 (rs1056836, Leu432Val) genes in patients with CRC cancer. It was also an attempt to identify the association between SNPs and CRC and its stage and grade at diagnosis. Methods: This case-control study was conducted in Kirkuk/Iraq, 200 patients with CRC and 200 cancer free control subjects were enrolled. Genomic DNA was extracted from venous blood samples and screened for SNPs using Restriction Fragment Length Polymorphism (RFLP) and confirmed by the direct DNA sequencing. Results: The reference genotype of CYP1A1 gene rs1048943 is AA. Both the AG and GG variants were significantly more frequent in the cancer group and associated with increased risks of CRC and its later stages (stages III and IV) and poor differentiation (p0.05) or with tumour stage or its grade (p>0.05). The GG genotype of CYP1B1 rs1056836 was the reference genotype. The CG and CC variants were not associated with increased risks of CRC (P>0.05) or its stage or grade except the CG genotype which was associated with poor differentiation (OR= 3.4, 95 % CI= 1.8 -6.5, P
- Subjects :
- 0301 basic medicine
Oncology
Adult
medicine.medical_specialty
cytochrome
Genotype
Colorectal cancer
CYP1B1
CYP1A1
Single-nucleotide polymorphism
Adenocarcinoma
Polymorphism, Single Nucleotide
03 medical and health sciences
Young Adult
0302 clinical medicine
Polymorphism (computer science)
Internal medicine
Genetic variation
Cytochrome P-450 CYP1A1
Medicine
Humans
Aged
Neoplasm Staging
Aged, 80 and over
business.industry
Cancer
General Medicine
Middle Aged
medicine.disease
030104 developmental biology
030220 oncology & carcinogenesis
Case-Control Studies
Cytochrome P-450 CYP1B1
Iraq
Restriction fragment length polymorphism
Neoplasm Grading
business
polymorphisms
Colorectal Neoplasms
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 2476762X and 15137368
- Volume :
- 22
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Asian Pacific Journal of Cancer Prevention : APJCP
- Accession number :
- edsair.doi.dedup.....5ec7eade461e90fd997c49b39952fa3a