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Long-term ingestion of reduced glutathione suppressed an accelerating effect of beef tallow diet on colon carcinogenesis in rats
- Source :
- Journal of Gastroenterology. 44:1026-1035
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- We have shown previously that long-term feeding of beef tallow increases colorectal cancer in rats. This study investigated the effects of enzymic antioxidant, reduced glutathione (GSH), on colon carcinogenesis in rats fed with beef tallow.Colon carcinogenesis was induced by intraperitoneal injection of azoxymethane (AOM) to rats. Rats were fed with 10% beef tallow supplemented with or without 1% GSH in drinking water. Aberrant crypt foci (ACF) and expression of beta-catenin in colonic mucosa were examined at 12 weeks. Cancers, related substances of oxidative stress and arachidonic acid cascade in plasma and normal colonic mucosa were determined at 44 weeks.GSH attenuated the number of ACF increased by beef tallow, but GSH had no influence on expression of beta-catenin increased by AOM. Incidence of colon cancer was no different with or without GSH, but GSH attenuated the number of colon cancers in each rat. GSH suppressed plasma malondialdehyde concentration. GSH increased GSH concentration and activities of catalase, glutathione peroxidase and superoxide dismutase in colonic mucosa, and decreased cyclooxygenase-2, prostaglandin E2 and thromboxane B2 levels.This study indicated that GSH suppressed the number of ACF, but the attenuation of colon carcinogenesis was limited to the number of colon cancers, although anti-oxidative effects and suppressive effects of arachidonic acid cascade were demonstrated by several indexes. These results suggested that colon carcinogenesis enhanced by beef tallow was partly caused by oxidative stress and arachidonic acid cascade, which were reduced by GSH.
- Subjects :
- Male
medicine.medical_specialty
Antioxidant
Colon
medicine.medical_treatment
Glutathione reductase
Azoxymethane
Antioxidants
Fats
Rats, Sprague-Dawley
Random Allocation
chemistry.chemical_compound
Intestinal mucosa
Tallow
Internal medicine
medicine
Animals
Intestinal Mucosa
beta Catenin
chemistry.chemical_classification
Arachidonic Acid
Glutathione peroxidase
Gastroenterology
food and beverages
Glutathione
Rats
Disease Models, Animal
Oxidative Stress
Cell Transformation, Neoplastic
Glutathione Reductase
Endocrinology
chemistry
Cyclooxygenase 2
Colonic Neoplasms
Precancerous Conditions
Aberrant crypt foci
Subjects
Details
- ISSN :
- 14355922 and 09441174
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....5ec36d82ddfcebea7250c17714ca7259