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Novel NG36/G9a gene products encoded within the human and mouse MHC class III regions
- Source :
- Mammalian Genome. 12:916-924
- Publication Year :
- 2001
- Publisher :
- Springer Science and Business Media LLC, 2001.
-
Abstract
- Previous annotation of the Class III region of the human Major Histocompatibility Complex (MHC) depicts NG36 as an independent gene, which lies immediately centromeric to the G9a gene. However, data presented in this report show that in human and mouse cells the NG36 and G9a genes are predominantly expressed within a single approximately 3.9-kbp transcript. Thus, the human NG36/G9a gene contains 28 exons (4 exons from the NG36 gene and 24 exons from the G9a gene), spans 17.938 kb, and encodes a 1210-amino acid polypeptide. In addition, a splice variant (NG36G9a-SPI), which lacks exon 10, was found to be coexpressed together with the full-length NG36/G9a transcript in both human and mouse cells. To aid functional characterization of the novel NG36/G9a gene-product, T7-epitope-tagged versions of the complete NG36/G9a protein or the G9a region alone (amino acids 210 to 1210) was transiently expressed in mammalian cells. Surprisingly, the sub-cellular distribution of the NG36/G9a-T7 and G9a-T7 proteins was found to be quite distinct. Whereas the G9a-T7 protein was observed in both the cytoplasm and the nucleus, the NG36/G9a-T7 protein was extensively concentrated within the nucleus. Also, the G9a-T7 protein frequently appeared marginalized at the nuclear periphery, while the NG36/G9a-T7 protein was generally found throughout the nucleoplasm. As such, it would appear that the NG36 domain plays a key role in controlling the sub-cellular distribution of the NG36/G9a protein.
- Subjects :
- Cytoplasm
DNA, Complementary
Transcription, Genetic
Biology
SYT1
Major Histocompatibility Complex
Jurkat Cells
Mice
SCN3A
Histocompatibility Antigens
Chlorocebus aethiops
HSPA2
Tumor Cells, Cultured
Genetics
Animals
Humans
Amino Acid Sequence
RNA, Messenger
RNA, Neoplasm
Cells, Cultured
HSPA9
TAF15
Cell Nucleus
Expressed Sequence Tags
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Alternative splicing
C4A
Exons
Histone-Lysine N-Methyltransferase
U937 Cells
Molecular biology
Neoplasm Proteins
Genes
COS Cells
AKT1S1
HeLa Cells
Subjects
Details
- ISSN :
- 14321777 and 09388990
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Mammalian Genome
- Accession number :
- edsair.doi.dedup.....5eb9013c8a067e1ff3b65466213f9f6a