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Methods for Assessing Scaffold Vascularization In Vivo
- Source :
- Skin Tissue Engineering ISBN: 9781493994724
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- The success of tissue engineering hinges on the rapid and sufficient vascularization of the neotissue. For efficient vascular network formation within three-dimensional (3D) constructs, biomaterial scaffolds that can support survival of endothelial cells as well as formation and maturation of a capillary network in vivo are highly sought after. Here, we outline a method to biofabricate 3D porous collagen scaffolds that can support extrinsic and intrinsic vascularization using two different in vivo animal models—the mouse subcutaneous implant model (extrinsic vascularization, capillary growth within the scaffold originating from host tissues outside the scaffold) and the rat tissue engineering chamber model (intrinsic vascularization, capillary growth within the scaffold derived from a centrally positioned vascular pedicle). These in vivo vascular tissue engineering approaches hold a great promise for the generation of clinically viable vascularized constructs. Moreover, the 3D collagen scaffolds can also be employed for 3D cell culture and for in vivo delivery of growth factors and cells.
- Subjects :
- Scaffold
Vascular pedicle
Chemistry
0206 medical engineering
Biomaterial
02 engineering and technology
021001 nanoscience & nanotechnology
020601 biomedical engineering
Cell biology
Porous collagen scaffolds
Neovascularization
3D cell culture
Tissue engineering
Cell culture
In vivo
Intrinsic vascularization
medicine
In vivo model
Extrinsic vascularization
medicine.symptom
0210 nano-technology
Subjects
Details
- ISBN :
- 978-1-4939-9472-4
- ISBNs :
- 9781493994724
- Database :
- OpenAIRE
- Journal :
- Skin Tissue Engineering ISBN: 9781493994724
- Accession number :
- edsair.doi.dedup.....5ea8918884903ad50f255fcec9051570