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Potentiated antitumor effects of APS001F/5-FC combined with anti-PD-1 antibody in a CT26 syngeneic mouse model
- Source :
- Bioscience, Biotechnology, and Biochemistry. 85:324-331
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- APS001F is a strain of Bifidobacterium longum genetically engineered to express cytosine deaminase that converts 5-fluorocytosine (5-FC) to 5-fluorouracil. In the present study, antitumor effects of APS001F plus 5-FC (APS001F/5-FC) in combination with anti-PD-1 monoclonal antibody were investigated using a CT26 syngeneic mouse model. Both of dosing of APS001F/5-FC before and after anti-PD-1 mAb in the combination dosing exhibited antitumor effects as well as prolonged survival over the nontreated control. The survival rate in the combination therapy significantly increased over the monotherapy with APS001F/5-FC and that with anti-PD-1 mAb. Regulatory T cells among CD4+ T cells in tumor decreased in the combination therapy, while the ratio of CD8+ T cells was maintained in all groups. Taken these results together, APS001F/5-FC not only demonstrates a direct antitumor activity, but also immunomodulatory effects once localized in the hypoxic region of the tumor, which allows anti-PD-1 mAb to exert potentiated antitumor effects.
- Subjects :
- 0301 basic medicine
Bifidobacterium longum
Combination therapy
medicine.drug_class
Programmed Cell Death 1 Receptor
Flucytosine
Antineoplastic Agents
CD8-Positive T-Lymphocytes
Pharmacology
Monoclonal antibody
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Mice
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
medicine
Animals
Molecular Biology
Survival rate
biology
Chemistry
Organic Chemistry
Cytosine deaminase
Anti pd 1
Antibodies, Monoclonal
General Medicine
biology.organism_classification
030104 developmental biology
030220 oncology & carcinogenesis
Syngeneic mouse
Genetic Engineering
CD8
Biotechnology
Subjects
Details
- ISSN :
- 13476947
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....5ea0ba12f9b3b9027bb073197127547d