Back to Search Start Over

High Developmental Rates of Mouse Oocytes Cryopreserved by an Optimized Vitrification Protocol: The Effects of Cryoprotectants, Calcium and Cumulus Cells

Authors :
Katsuyoshi Fujiwara
Naomi Kashiwazaki
Junya Ito
Natsuki Kohaya
Source :
Journal of Reproduction and Development. 57:675-680
Publication Year :
2011
Publisher :
Japanese Society of Animal Reproduction, 2011.

Abstract

Unfertilized oocytes are one of the most desired germ cell stages for cryopreservation because these cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, in general, the fertility and developmental ability of cryopreserved oocytes are still low. The aim of the present study was to improve vitrification of mouse oocytes. First, the effects of calcium and cryoprotectants, dimethyl sulfoxide and ethylene glycol (EG), in vitrification medium on survival and developmental ability of vitrified oocytes were evaluated. Oocytes were vitrified by a minimal volume cooling procedure using different cryoprotectants. Most of the vitrified oocytes were morphologically normal after warming, but their fertility and development were low independently of calcium and cryoprotectants. Second, the effect of cumulus cells on ability of oocytes to be fertilized and develop in vitro was examined. The fertility and developmental ability of denuded oocytes (DOs) after IVF were reduced compared with cumulus-oocyte complexes (COCs) both in fresh and cryopreserved groups. Vitrified COCs showed significantly (P

Details

ISSN :
13484400 and 09168818
Volume :
57
Database :
OpenAIRE
Journal :
Journal of Reproduction and Development
Accession number :
edsair.doi.dedup.....5e974ce67208e63f29ebf59b9bee55fd
Full Text :
https://doi.org/10.1262/jrd.11-066h