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High Developmental Rates of Mouse Oocytes Cryopreserved by an Optimized Vitrification Protocol: The Effects of Cryoprotectants, Calcium and Cumulus Cells
- Source :
- Journal of Reproduction and Development. 57:675-680
- Publication Year :
- 2011
- Publisher :
- Japanese Society of Animal Reproduction, 2011.
-
Abstract
- Unfertilized oocytes are one of the most desired germ cell stages for cryopreservation because these cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, in general, the fertility and developmental ability of cryopreserved oocytes are still low. The aim of the present study was to improve vitrification of mouse oocytes. First, the effects of calcium and cryoprotectants, dimethyl sulfoxide and ethylene glycol (EG), in vitrification medium on survival and developmental ability of vitrified oocytes were evaluated. Oocytes were vitrified by a minimal volume cooling procedure using different cryoprotectants. Most of the vitrified oocytes were morphologically normal after warming, but their fertility and development were low independently of calcium and cryoprotectants. Second, the effect of cumulus cells on ability of oocytes to be fertilized and develop in vitro was examined. The fertility and developmental ability of denuded oocytes (DOs) after IVF were reduced compared with cumulus-oocyte complexes (COCs) both in fresh and cryopreserved groups. Vitrified COCs showed significantly (P
- Subjects :
- Ethylene Glycol
Cryoprotectant
medicine.medical_treatment
Reproductive technology
Intracytoplasmic sperm injection
Cryopreservation
Andrology
Mice
Cryoprotective Agents
medicine
Animals
Dimethyl Sulfoxide
Vitrification
Blastocyst
Cumulus Cells
In vitro fertilisation
Chemistry
Oocyte
medicine.anatomical_structure
Oocytes
Calcium
Female
Animal Science and Zoology
Subjects
Details
- ISSN :
- 13484400 and 09168818
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Journal of Reproduction and Development
- Accession number :
- edsair.doi.dedup.....5e974ce67208e63f29ebf59b9bee55fd
- Full Text :
- https://doi.org/10.1262/jrd.11-066h