Back to Search
Start Over
TM1-IR680 peptide for assessment of surgical margin and lymph node metastasis in murine orthotopic model of oral cancer
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Treatment outcome after surgical removal in oral carcinoma is poor due to inadequate methodologies available for marking surgical margins. Even though some methodologies for intraoperative margin assessment are under clinical and preclinical trials for other solid tumours, a promising modality for oral cancer surgery is not developed. Fluorescent-based optical imaging using Near Infrared (NIR) dyes tagged to tumour specific target will be an optimal tool for this purpose. One such target, Gastrin Releasing Peptide Receptor (GRPR) was selected for the study, and its binding peptide, TM1-IR680, was tested for its efficacy for surgical margin prediction in murine orthotopic model of oral cancer, derived from primary samples. Here, for the first time in a preclinical analysis, we show that the size and margin of oral cancer can be predicted, as revealed by 3D-imaging. Interestingly, the peptide was sensitive enough to detect lymph nodes that harboured dispersed tumour cells before colonization, which was impossible to identify by conventional histopathology. We recommend the use of TM1-NIR dyes alone or in combination with other technologies to improve the clinical outcome of oral cancer surgery.
- Subjects :
- 0301 basic medicine
Oncology
medicine.medical_specialty
Surgical margin
Peptide
Article
Mice
03 medical and health sciences
0302 clinical medicine
Text mining
Mice, Inbred NOD
Cell Line, Tumor
Internal medicine
medicine
Gastrin-releasing peptide receptor
Carcinoma
Animals
Humans
chemistry.chemical_classification
Multidisciplinary
business.industry
Optical Imaging
Cancer
Neoplasms, Experimental
medicine.disease
Neoplasm Proteins
Receptors, Bombesin
030104 developmental biology
chemistry
Lymphatic Metastasis
030220 oncology & carcinogenesis
Mouth Neoplasms
Histopathology
Lymph
Carrier Proteins
Peptides
business
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....5e71a2ff810905d00d1da8266003dd18