Back to Search
Start Over
Activity of the Principal Cells of the Olfactory Bulb Promotes a Structural Dynamic on the Distal Dendrites of Immature Adult-Born Granule Cells via Activation of NMDA Receptors
- Source :
- The Journal of Neuroscience. 34:1748-1759
- Publication Year :
- 2014
- Publisher :
- Society for Neuroscience, 2014.
-
Abstract
- The adult olfactory bulb is continuously supplied with neuronal precursors that differentiate into granule and periglomerular cells. Little is known about the structural dynamic of adult-born granule cells (GCs) at their different maturational stages, the mechanisms controlling the integration of new neurons into the pre-existing neuronal circuitry, or the role of principal cell activity in these processes. We used two-photon time-lapse imaging to reveal a high level of filopodia formation and retraction on the distal dendrites of adult-born GCs at their early maturational stages. This dynamic decreased as the adult-born interneurons matured. Filopodia formation/retraction on the dendrites of adult-born GCs at the early maturational stages depended on the activation of NMDA receptors (NMDARs). The stimulation of mitral cells using a pattern that mimics activity of these principal neurons to odor presentation promotes the NMDAR-dependent filopodia dynamic of adult-born GCs during their early but not late maturational stages. Moreover, NMDA iontophoresis was sufficient to induce the formation of new filopodia on the distal dendrites of immature adult-born GCs. The maturation of adult-born interneurons was accompanied by a progressive hyperpolarization of the membrane potential and an increased Mg2+block of NMDARs. Decreasing the extracellular Mg2+concentration led to filopodia formation on the dendrites of mature adult-born GCs following NMDA iontophoresis. Our findings reveal an increased structural dynamic of adult-born GCs during the early stages of their integration into the mouse bulbar circuitry and highlight a critical period during which the principal cells' activity influences filopodia formation/retraction on the dendrites of interneurons.
- Subjects :
- Male
N-Methylaspartate
Patch-Clamp Techniques
Time Factors
Green Fluorescent Proteins
Stimulation
Biology
Receptors, N-Methyl-D-Aspartate
Time-Lapse Imaging
Statistics, Nonparametric
Membrane Potentials
Mice
Excitatory Amino Acid Agonists
Extracellular
Animals
Pseudopodia
Neurons
Membrane potential
Iontophoresis
General Neuroscience
Neurogenesis
Gene Transfer Techniques
Excitatory Postsynaptic Potentials
Dendrites
Articles
Olfactory Bulb
Olfactory bulb
Mice, Inbred C57BL
Adult Stem Cells
nervous system
Odorants
NMDA receptor
Excitatory Amino Acid Antagonists
Neuroscience
Filopodia
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....5e6e71d266f7c2fa197f2af9ee50bc9f
- Full Text :
- https://doi.org/10.1523/jneurosci.3013-13.2014