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Berberine: A nematocidal alkaloid from Argemone mexicana against Strongyloides venezuelensis
- Source :
- Experimental Parasitology. 220:108043
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Strongyloidiasis is a parasitosis that represents a public health problem, in tropical regions. The present study aimed to investigate the anthelmintic effects of several extracts of Argemone mexicana, as well as its main component berberine (Ber) against the third-stage larvae (L3) of Strongyloides venezuelensis in-vitro experiments. Also, the anti-hemolytic activity of the extract, fractions, and Ber were tested in human erythrocytes. A dose-response anthelminthic bioassay demonstrated Ber as the most effective component, followed by methanolic subfraction (Fr3) and finally the crude extract of A. mexicana (Am) showing LC50 response values of 1.6, 19.5, and 92.1 μg/mL, at 96 h respectively. Also, Am, Fr3, and Ber did not produce significant hemolysis against human erythrocytes (p ≤ 0.05). Am and Fr3 showed erythrocyte protection effect capacity at the membrane level (p ≤ 0.05). Furthermore, Ber was found to have an antioxidant activity of 168.18 μg/mL. According to the results, the Fr3 of A. mexicana, and particularly Ber, exhibited potent in-vitro effects against L3 of S. venezuelensis, without hemolytic activity against human erythrocytes and presented good antioxidant capacity. In conclusion, the extracts of A. mexicana and the main component have activity against S. venezuelensis, nevertheless, further studies are required to elucidate the mechanism of action.
- Subjects :
- Male
Erythrocytes
Antioxidant
Berberine
medicine.medical_treatment
Immunology
Hemolysis
Argemone mexicana
Lethal Dose 50
Feces
chemistry.chemical_compound
Strongyloides
medicine
Animals
Humans
Bioassay
Anthelmintic
Rats, Wistar
Anthelmintics
Analysis of Variance
Dose-Response Relationship, Drug
Plant Stems
biology
Traditional medicine
Plant Extracts
Alkaloid
General Medicine
medicine.disease
biology.organism_classification
Rats
Plant Leaves
Infectious Diseases
Mechanism of action
chemistry
Larva
Strongyloidiasis
Argemone
Biological Assay
Parasitology
medicine.symptom
medicine.drug
Subjects
Details
- ISSN :
- 00144894
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- Experimental Parasitology
- Accession number :
- edsair.doi.dedup.....5e66c2ab96a2c5ed7d671e3e429037e3
- Full Text :
- https://doi.org/10.1016/j.exppara.2020.108043