Back to Search
Start Over
Analysis of DNA methylation differences in gonads of the large yellow croaker
- Source :
- Gene. 749:144754
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- DNA methylation is an essential epigenetic modification that significantly regulates gene expression during development and differentiation. In this study, genome-wide methylation analysis of different gonads of the large yellow croaker was performed using whole-genome bisulfite sequencing (WGBS), which has characterized DNA methylation patterns in gonad tissue and identified candidate regions for future studies. Clustering analysis revealed that male and neomale methylation patterns were close compared to female. Based on KEGG pathway analysis of differentially methylated genes, we obtained signaling pathways related to gonadal development. We further investigated the methylation status of previously reported sex determination genes, and found that these genes showed different methylation status in three types of gonads, which may provide important clues to reveal the sex determination genes in the large yellow croaker. Furthermore, combined with transcriptome analysis, we found 7 sex-related genes in three comparison groups where expression negatively correlated with methylation.
- Subjects :
- Male
0301 basic medicine
endocrine system
Bisulfite sequencing
Biology
Epigenesis, Genetic
Transcriptome
Cytosine
03 medical and health sciences
0302 clinical medicine
Gene expression
Genetics
Animals
RNA-Seq
Epigenetics
Gonads
Promoter Regions, Genetic
Gene
Sequence Analysis, DNA
General Medicine
Methylation
DNA Methylation
Sex Determination Processes
Perciformes
030104 developmental biology
030220 oncology & carcinogenesis
DNA methylation
Female
Development of the gonads
Transcription Factors
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 749
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....5e50fda924bdbcf7577029fb4b27da43