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Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells
- Source :
- Anticancer research. 39(11)
- Publication Year :
- 2019
-
Abstract
- Background/aim For patients undergoing cancer surgery, the risk for cancer progression is enhanced during the perioperative period. To what extent the type of anesthetic can affect the metastatic process and finally the outcome of patients with cancer is under debate. For this reason, the aim of this study was to investigate the effects of the volatile anesthetics sevoflurane and desflurane on colon cancer cells in vitro. Materials and methods SW480 colon carcinoma cells were exposed for 3 or 6 h to sevoflurane (1 or 2.5 vol%) or desflurane (6 or 12 vol%). Cell cycle distribution was analyzed by flow cytometry after a 24-72 h recovery and apoptosis was detected by annexin V staining after a 0-48 h recovery. Viability was tested by measuring ATP content after 0 and 24 h recovery. Results Treatment with sevoflurane or desflurane caused no or only slight changes in cell-cycle distribution and apoptosis rate. Desflurane at 12vol% significantly reduced cell viability by 17±25% and 11±22% after 3 and 6 h incubation and 24 h recovery, respectively, while 2.5 vol% sevoflurane slightly increased viability. Conclusion At clinically relevant concentrations, sevoflurane and desflurane had only slight effects on SW480 colon cancer cells in vitro.
- Subjects :
- Cancer Research
Colorectal cancer
Apoptosis
Pharmacology
In Vitro Techniques
Sevoflurane
Flow cytometry
03 medical and health sciences
Desflurane
0302 clinical medicine
Tumor Cells, Cultured
Medicine
Humans
Viability assay
Cell Proliferation
medicine.diagnostic_test
business.industry
Cell Cycle
Cancer
General Medicine
medicine.disease
Oncology
030220 oncology & carcinogenesis
Anesthetic
Anesthetics, Inhalation
Colonic Neoplasms
business
medicine.drug
Subjects
Details
- ISSN :
- 17917530
- Volume :
- 39
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Anticancer research
- Accession number :
- edsair.doi.dedup.....5e477d9c32ce34dcee5c8f9925cbacb3