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Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants

Authors :
Young Teck Kim
C. H. Ha
Chan Wha Kim
Hyo Ihl Chang
K. H. Lim
S. T. Lim
Source :
Applied Microbiology and Biotechnology. 58:370-377
Publication Year :
2002
Publisher :
Springer Science and Business Media LLC, 2002.

Abstract

The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-beta-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkalysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of beta-(1,6)-D-linkage than that from the wild-type.

Details

ISSN :
14320614 and 01757598
Volume :
58
Database :
OpenAIRE
Journal :
Applied Microbiology and Biotechnology
Accession number :
edsair.doi.dedup.....5e403087a30190cda90ab3b719baff66