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Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants
- Source :
- Applied Microbiology and Biotechnology. 58:370-377
- Publication Year :
- 2002
- Publisher :
- Springer Science and Business Media LLC, 2002.
-
Abstract
- The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-beta-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkalysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of beta-(1,6)-D-linkage than that from the wild-type.
- Subjects :
- Chromatography, Gas
Magnetic Resonance Spectroscopy
Chemical structure
Mutant
Saccharomyces cerevisiae
Alkalies
Biology
Polysaccharide
Applied Microbiology and Biotechnology
Cell wall
Cell Wall
Ultraviolet light
Glucans
Chromatography, High Pressure Liquid
Glucan
chemistry.chemical_classification
Molecular Structure
Glucan Endo-1,3-beta-D-Glucosidase
Polysaccharides, Bacterial
General Medicine
biology.organism_classification
Yeast
Solubility
chemistry
Biochemistry
Mutation
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....5e403087a30190cda90ab3b719baff66