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Determination of 77 Multiclass Pesticides and Their Metabolitesin Capsicum and Tomato Using GC-MS/MS and LC-MS/MS

Authors :
Harischandra Naik Rathod
Bheemanna Mallappa
Pavankumar Kamin
Udaykumar Nidoni
Bheemsain Rao Kishan Rao Desai
Chandra Sekhara Reddy Vennapusa
Saroja Narsing Rao
Paramasivam Mariappan
Pallavi Malenahalli Sidramappa
Source :
Molecules, Volume 26, Issue 7, Molecules, Vol 26, Iss 1837, p 1837 (2021)
Publication Year :
2021
Publisher :
Multidisciplinary Digital Publishing Institute, 2021.

Abstract

A quick, sensitive, and reproducible analytical method for the determination of 77 multiclass pesticides and their metabolites in Capsicum and tomato by gas and liquid chromatography tandem mass spectrometry was standardized and validated. The limit of detection of 0.19 to 10.91 and limit of quantification of 0.63 to 36.34 µg·kg−1 for Capsicum and 0.10 to 9.55 µg·kg−1 (LOD) and 0.35 to 33.43 µg·kg−1 (LOQ) for tomato. The method involves extraction of sample with acetonitrile, purification by dispersive solid phase extraction using primary secondary amine and graphitized carbon black. The recoveries of all pesticides were in the range of 75 to 110% with a relative standard deviation of less than 20%. Similarly, the method precision was evaluated interms of repeatability (RSDr) and reproducibility (RSDwR) by spiking of mixed pesticides standards at 100 µg·kg−1 recorded anRSD of less than 20%. The matrix effect was acceptable and no significant variation was observed in both the matrices except for few pesticides. The estimated measurement uncertainty found acceptable for all the pesticides. This method found suitable for analysis of vegetable samples drawn from market and farm gates.

Details

Language :
English
ISSN :
14203049
Database :
OpenAIRE
Journal :
Molecules
Accession number :
edsair.doi.dedup.....5e3cff48b47143867b2fcff73bc447aa
Full Text :
https://doi.org/10.3390/molecules26071837