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Plant potassium nutrition in ectomycorrhizal symbiosis: properties and roles of the three fungal TOK potassium channels in Hebeloma cylindrosporum
- Source :
- Environmental Microbiology, Environmental Microbiology, Wiley-Blackwell, 2018, 20 (5), pp.1873-1887. ⟨10.1111/1462-2920.14122⟩, Environmental Microbiology 5 (20), 1873-1887. (2018), Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2018, 20 (5), pp.1873-1887. ⟨10.1111/1462-2920.14122⟩
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Ectomycorrhizal fungi play an essential role in the ecology of boreal and temperate forests through the improvement of tree mineral nutrition. Potassium (K+ ) is an essential nutrient for plants and is needed in high amounts. We recently demonstrated that the ectomycorrhizal fungus Hebeloma cylindrosporum improves the K+ nutrition of Pinus pinaster under shortage conditions. Part of the transport systems involved in K+ uptake by the fungus has been deciphered, while the molecular players responsible for the transfer of this cation towards the plant remain totally unknown. Analysis of the genome of H. cylindrosporum revealed the presence of three putative tandem-pore outward-rectifying K+ (TOK) channels that could contribute to this transfer. Here, we report the functional characterization of these three channels through two-electrode voltage-clamp experiments in oocytes and yeast complementation assays. The expression pattern and physiological role of these channels were analysed in symbiotic interaction with P. pinaster. Pine seedlings colonized by fungal transformants overexpressing two of them displayed a larger accumulation of K+ in shoots. This study revealed that TOK channels have distinctive properties and functions in axenic and symbiotic conditions and suggested that HcTOK2.2 is implicated in the symbiotic transfer of K+ from the fungus towards the plant.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Potassium Channels
Saccharomyces cerevisiae
Fungus
maritime pine
absorption du potassium
champignon mycorhizien
01 natural sciences
Microbiology
nutrition minérale
Ectosymbiosis
03 medical and health sciences
Symbiosis
Mycorrhizae
Botany
Hebeloma
ion potassium
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Axenic
Ecology, Evolution, Behavior and Systematics
Minerals
Vegetal Biology
pinus pinaster
shaker potassium channel
biology
fungi
Biological Transport
15. Life on land
Pinus
biology.organism_classification
Yeast
Complementation
hebeloma cylindrosporum
030104 developmental biology
Seedlings
Potassium
Pinus pinaster
canaux potassiques shaker
Plant nutrition
Biologie végétale
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14622920 and 14622912
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....5e38a874c544c5b43488a62043cba8ac