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Increased matrix metalloproteinase-9 secretion in short-term tissue cultures of prostatic tumor cells
- Source :
- International Journal of Cancer. 69:386-393
- Publication Year :
- 1996
- Publisher :
- Wiley, 1996.
-
Abstract
- We have examined the expression of 2 tumor-associated metalloproteinases, MMP-2 and MMP-9, in 48 primary cultures of prostatic carcinoma (PRCA) and 33 cultures of benign prostatic hyperplasia (BPH). PRCA cultures secrete significantly more MMP-9 than their benign counterparts. Secreted MMP-2 did not differ significantly in cultures but was lower in PRCA cultures. Two cultures of benign origin exhibited high MMP-9 secretion and growth patterns consistent with a malignancy. Both cases were followed and successively re-evaluated histologically and rediagnosed as organ-confined PRCA. MMP expression in culture may be of predictive value in the identification of incidental PRCA. MMP-9 secretion and its ratio with MMP-2 were highest in epithelial cultures from invasive, metastatic tumors when compared both to disease confined to prostate gland and to locally extensive disease. MMP-9 secretion was greatest also in cultures derived from tissues of high Gleason histological grade. Active MMP-9 species were detected in 15 cultures (31%) of PRCA. Active MMP-2 species were observed in cultures of both BPH and PRCA origin in almost the same amounts. Although average levels were not significantly different, as a ratio to proform species, a significant elevation was observed in cultures of PRCA origin. We propose, therefore, that an elevated expression of MMP-9 and a high ratio of MMP-9 to MMP-2 in short-term prostate epithelial cultures is of potential diagnostic and prognostic significance.
- Subjects :
- Male
Cancer Research
Pathology
medicine.medical_specialty
Matrix metalloproteinase
Biology
urologic and male genital diseases
Malignancy
medicine.disease_cause
Epithelium
Settore MED/24 - Urologia
Diagnosis, Differential
Dose-Response Relationship
Tissue culture
Prostate
Diagnosis
Tumor Cells, Cultured
medicine
Carcinoma
Humans
controlled study
human
tissue culture
Neoplasm Staging
Cultured
Dose-Response Relationship, Drug
Epidermal Growth Factor
human cell
gelatinase
article
Metalloendopeptidases
Prostatic Neoplasms
gelatinase b
metalloproteinase
major clinical study
male
priority journal
prostate cancer
Hyperplasia
medicine.disease
Tumor Cells
medicine.anatomical_structure
Oncology
Differential
Drug
Carcinogenesis
Subjects
Details
- ISSN :
- 10970215 and 00207136
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi.dedup.....5e1157a618666d514eda53054cb3dcfe