Back to Search
Start Over
Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels
- Source :
- One Health, One Health, Vol 7, Iss, Pp-(2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Shiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter collected from 163 dromedaries on a large ranch were screened for the presence of stx1 and stx2. STEC strains were isolated and serotyped. Isolates were subjected to PCR for the subtyping of stx genes and for the detection of eae and ehx. In addition, whole genome sequencing (WGS) was carried out to detect further virulence genes and to determine the multilocus sequence types (MLST). Antimicrobial resistance profiles were determined by disk diffusion.STEC was isolated from 20 (12.3%) of the fecal samples. Thereof, nine (45%) isolates were STEC O156:H25, three (15%) isolates typed STEC O43:H2. The remaining isolates occurred as single serotypes or were O non-typeable. Eleven (55%) of the isolates harboured stx2a, nine (45%) eae, and 14 (70%) ehx, respectively. WGS revealed the presence of iss in 16 (80%), subAB in four (20%) and astA in two (10%) of the isolates, Furthermore, espA, tccP, nleA, nleB, tccP, and tir were found exclusively among STEC O156:H25.Eleven different sequence types (ST) were detected. The most prominent was ST300/ST5343, which comprised STEC O156:H25. All STEC isolates were pan susceptible to a panel of 16 antimicrobial agents. Overall, the results indicate that dromedary camels in Kenya may be reservoirs of STEC, including serotypes possessing virulence markers associated to disease in humans, such as STEC O156:H25. STEC in camels may represent a health hazard for humans with close contact to camels or to consumers of camel derived foodstuffs, such as unpasteurised camel milk. Keywords: Camels, STEC, Serotypes, Virulence genes, Whole genome sequencing
- Subjects :
- Serotype
Serotypes
animal diseases
Virulence
610 Medicine & health
Biology
medicine.disease_cause
Microbiology
fluids and secretions
Camels
STX2
medicine
Camel milk
Escherichia coli
10082 Institute of Food Safety and Hygiene
Feces
lcsh:R5-920
Antiinfective agent
Public Health, Environmental and Occupational Health
2739 Public Health, Environmental and Occupational Health
2725 Infectious Diseases
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
STEC
Infectious Diseases
Virulence genes
Whole genome sequencing
570 Life sciences
biology
bacteria
Multilocus sequence typing
lcsh:Medicine (General)
Research Paper
Subjects
Details
- ISSN :
- 23527714
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- One Health
- Accession number :
- edsair.doi.dedup.....5e0d54b7d4040ee8955e87c83008af27