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Nuclear morphometry as an adjunct to cytopathologic examination of endometrial brushings on LBC samples: A prospective approach to combined evaluation in endometrial neoplasms and look alikes
- Source :
- Cytopathology : official journal of the British Society for Clinical CytologyReferences. 32(1)
- Publication Year :
- 2020
-
Abstract
- Objective In this study, we aimed to retrospectively investigate and confirm whether atypical nuclear findings in endometrial cytology are useful when assessed by image morphometry in liquid-based cytology (LBC) and compared with microscopic evaluation. Methods In total, 53 cases were selected for this study, including 11 presenting proliferative endometrium, 12 with surface papillary syncytial change with endometrial glandular and stromal breakdown (EGBD-SPSC), 10 endometrioid carcinoma grade 1 (G1-EEC), 10 EEC grade 3 (G3-EEC), and 10 endometrial serous carcinomas (ESC). Nuclear image morphometry for nuclear geometric features (area, grey value, aspect ratio, internuclear distance, nucleolar diameter) was performed using ImageJ computer software. For assessing nucleoli, 3861 nuclei were measured, and for nuclear findings, except for nucleoli, 4036 nuclei were measured in total. Results (a) Compared with G1-EEC, G3-EEC and ESC presented a marked increase in all six parameters (nuclear enlargement, anisonucleosis, nuclear shade, nuclear shape, irregularity of nuclear arrangement, and nucleolar size). (b) EGBD-SPSC presented a marked increase in two parameters (nuclear shade, nuclear shape) when compared with G1/G3-EEC and ESC. (c) Compared with EGBD-SPSC, EEC and ESC demonstrated a marked increase in nucleolar size (≥2.0 μm). (d) ESC presented a marked increase in nucleolar size (≥3.0 μm) when compared with G3-EEC. Conclusions Here we confirmed that atypical nuclear findings evaluated by image morphometry are as useful as microscopic evaluations in endometrial cytology. We believe that the objective evaluation of nucleolar size could contribute to an accurate diagnosis of endometrial-LBC samples.
- Subjects :
- Pathology
medicine.medical_specialty
Histology
Nucleolus
Cytodiagnosis
030209 endocrinology & metabolism
Pathology and Forensic Medicine
03 medical and health sciences
Endometrium
0302 clinical medicine
Cytology
medicine
Carcinoma
Biomarkers, Tumor
Humans
Nuclear atypia
Prospective Studies
Retrospective Studies
Cell Nucleus
business.industry
General Medicine
medicine.disease
Nuclear shape
Cystadenocarcinoma, Serous
Endometrial Neoplasms
stomatognathic diseases
Endometrial cytology
Serous fluid
030220 oncology & carcinogenesis
Liquid-based cytology
Female
business
Carcinoma, Endometrioid
Subjects
Details
- ISSN :
- 13652303
- Volume :
- 32
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cytopathology : official journal of the British Society for Clinical CytologyReferences
- Accession number :
- edsair.doi.dedup.....5df3fdf764209919f996fe339dcb2466