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Genotyping of Candida albicans using length fragment and high-resolution melting analyses together with minisequencing of a polymorphic microsatellite locus
- Source :
- Journal of Microbiological Methods, Journal of Microbiological Methods, Elsevier, 2010, 80 (3), pp.306-309. ⟨10.1016/j.mimet.2010.01.002⟩, Journal of Microbiological Methods, 2010, 80 (3), pp.306-309. ⟨10.1016/j.mimet.2010.01.002⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- International audience; Microsatellite length polymorphism (MLP) typing is a PCR-based method used for genotyping of the diploid yeast Candida albicans. However, MLP is subject to homoplasia which can hamper the accuracy of the results. We combined fragment length analysis, high-resolution DNA melting (HRM) analysis, and SNaPshot minisequencing after a single amplification of the CDC3 locus to study 95 epidemiologically independent C. albicans isolates. HRM analysis for a given electrophoretic group led to a maximum of three different curves due to the presence of a SNP upstream of the tandem repeat which could be characterized using the SNaPshot assay. The combination of the three methods had a discriminatory index of 0.88 in complete congruence with previous MLP typing (Mantel test R=0.99, P
- Subjects :
- Microbiology (medical)
Genotyping
MESH: Sequence Analysis, DNA
Locus (genetics)
MESH: Nucleic Acid Denaturation
Biology
Nucleic Acid Denaturation
Polymerase Chain Reaction
Microbiology
High Resolution Melt
03 medical and health sciences
High-resolution melting
Tandem repeat
Genotype
Candida albicans
MESH: Polymorphism, Genetic
Humans
Typing
MESH: Genetic Variation
DNA, Fungal
Molecular Biology
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
030304 developmental biology
Genetics
Single-nucleotide polymorphism
0303 health sciences
Polymorphism, Genetic
MESH: Humans
030306 microbiology
MESH: Candida albicans
Candidiasis
Genetic Variation
MESH: Polymerase Chain Reaction
Sequence Analysis, DNA
MESH: Candidiasis
MESH: DNA, Fungal
Genetic marker
MESH: Genome, Fungal
Microsatellite
MESH: Microsatellite Repeats
Genome, Fungal
Microsatellite length polymorphism
Microsatellite Repeats
Subjects
Details
- Language :
- English
- ISSN :
- 01677012 and 18728359
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiological Methods, Journal of Microbiological Methods, Elsevier, 2010, 80 (3), pp.306-309. ⟨10.1016/j.mimet.2010.01.002⟩, Journal of Microbiological Methods, 2010, 80 (3), pp.306-309. ⟨10.1016/j.mimet.2010.01.002⟩
- Accession number :
- edsair.doi.dedup.....5dd9b817b0f53ef352d926a4ea75d963